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We narrowed to 1,011 results for: ARC

Showing: 421 - 440 of 1011 results
  1. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ...visibly complex - and unique - adult. My initial research directions followed that avenue with projects ...interests and skills than what fits for applied research. I focus in general terms on a couple of very ...teaching – often explaining a technical piece of research to others outside that specialty, who may be other...professional meetings, societies, networks just as do research scientists. There we meet others in the scientific...
  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively...believe that these results will be beneficial to the research community.    So, why are we so excited? For starters...inventory and more time providing new materials to the research community. References 1. Coroadinha, A. S., ...
  3. New and Upcoming Viral Vectors - December 2019

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    ...H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns...sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters... viral protocols and videos Find AAVs for your research ...
  4. Single-cell tracking of lineage and identity with CellTag

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    Blog Post
    ...tagging method that labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6...Guo, Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics...generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in model organisms...
  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post
    ...partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent...rather than cyan and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation...function of the protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both...posts about choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org...
  6. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...also bring benefits to researchers conducting basic or early pre-clinical research. However, standard terms...concept of gRNA design is still challenging for researchers using CRISPR, and that standardizing and improving...resources and publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say...
  7. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    Blog Post
    ...Although plasmids started as a somewhat niche area of research, they are now seen as an ubiquitous tool that ...little bit easier for scientists to conduct their research! Since their discovery in the 1950s, plasmids ...have greatly impacted many areas of biological research and have been key in advancing our knowledge in... Marcy Patrick...
  8. Extracurricular Activities for a Strong Science Career Path

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    ...downward trend in funding and space in academic research labs has more students looking for other options...perspectives in science and engineering Looking at your research from a broader viewpoint may expose which experiments...extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking a break... skill sets Taking the time to identify and research 2 – 3 potential professions will highlight which...
  9. Deaminet 2026: Breakthroughs in Base Editing, Deaminase Biology, and Therapeutic Translation

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    Blog Post
    ...Palm Springs in late January, brought together researchers studying deaminase enzymes across disciplines... dissecting mutational processes, to medical researchers advancing base and prime editing toward the clinic...rapid, worldwide open access and maximize your research’s impact! This blog post was written by Addgenie...base editing efficiency in ABE8e. Nucleic Acids Research, 52(22):13931-13944. https://doi.org/10.1093/nar...off-target activity induced by prime editors. Genome Research, 35(9), 2064–2075. https://doi.org/10.1101/gr.280164.124...
  10. Antibody Validation for Flow Cytometry

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    ...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi....
  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...increasingly more cost effective and precise, researchers are now using it to investigate potential therapeutics...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...correct disease hadn’t yet been realized. The researchers chose to explore its potential in mice with a...known as Crygc. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...
  12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post
    ...development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found that affect...
  13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...method. Read on to learn how to use it in your research! The starting point: Ligation-independent cloning...only one type of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless...
  14. Viral Vectors 101: Viral Applications

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    Blog Post
    ... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...overexpression of all human transcription factors, barcoded libraries to trace cell lineage, and so much more...
  15. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... Optogenetics Plasmids for Use in Neuroscience Research Neuroscience covers many subfields When I first...neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect Alzheimer's...projects using biological tools. In addition to the researcher using sequencing technologies to study microbiome...making more AAVs and educational content to help researchers use these great tools. If you’re just getting...
  16. A Deep Dive into BioBuilder

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    Blog Post
    ...Drawing on current questions being asked in MIT’s research labs, BioBuilder inspires students and teachers...malleable curriculum, BioBuilder takes cutting-edge research projects in synthetic biology and transforms them...and watching animations that present a specific research problem and experiment to tackle. Next it’s off...and provides a nationally recognized platform in March to exhibit projects at any stage of completion, ...
  17. Hot Plasmids - February 2022

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    Blog Post
    ...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic...
  18. Antibodies 101: Monoclonal Antibodies

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    ...-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies...https://doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies...
  19. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post
    ...expression of recombinant proteins for industrial or research purposes requires stable host cells (the simplest...chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that...commercial operators, government agencies, active researchers, as well as fellow iGEM teams. We were the proud...more productive and collaborative environment for research.  We’ve also involved ourselves in extensive outreach...
Showing: 421 - 440 of 1011 results