We narrowed to 591 results for: ARL

1. 7 Tips to Secure a STEAM Internship This Summer

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   Blog Post

   ...Addgene blog Check out other tools and resources for early career researchers Applying for jobs? Check out ...

2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as APEX2. What’s more, since the enzymes...

3. A Deep Dive into BioBuilder

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   ...teacher professional development program that trains nearly 100 teachers each year, with workshops held at ...

4. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical feasibility...

5. Addgene’s Viral Service - Why Virus? Why Now?

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   ...fulfilling our mission for an even bigger impact. In early 2016 we surveyed hundreds of scientists about various...

6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...most abundant molecules in a cell, this system is clearly also robust enough to ensure high levels of gRNA...

7. CRISPR 101: Non-Homologous End Joining

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   ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...test was the perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR...

9. 3 Challenges in Plant Synthetic Biology

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   ...challenge associated with molecular plant parts, particularly for applied projects intended for commercialization...

10. Plasmids 101: Yeast Vectors

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    Blog Post

    ...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast...

11. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    ...knock-out mice, but for various reasons it is not nearly as efficient in human stem cells. What genome editing...

12. Seven Tips for Using LinkedIn as a Scientist

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    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...

13. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...having difficulties in amplifying a template, particularly from a large genome, you can recheck your primer...

14. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression...

15. RaPID Detection of RNA-protein Interactions

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    ...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations...

16. dTAG - You're it!

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    ...proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...

17. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...and quantitative analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found ...

18. Antibodies 101: Conjugation

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    ...touched on fluorophores as antibody conjugates in an earlier post, where you can find some additional tips for...

19. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...et al., Frontiers in Genome Editing). This is particularly important in experiments in which viability ...

20. What's New in CRISPR - November 2021

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    Blog Post

    ...Building upon the PE2 and PE3 systems of their earlier work, they developed a system in which the cellular...