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  1. Typing CRISPR Systems

    Type
    Blog Post
    ...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...
  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...
  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...
  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...
  5. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...point on the axis determined by the researcher, based on their controls. You can usually tell where this...Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to...
  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...results (Sinnett et al., 2017; Powers et al., 2019). Based on the work of the consortium, two gene therapy ...guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into the hippocampus...iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References: Amir ...
  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...environment. The “how many?” question lies at the base of all these questions as there is not particularly...for their buck - make them demand more services based on what they're spending. Increase the number of...
  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...cells are classified as pluripotent or multipotent, based on their ability to generate different cell lineages...Cell research. You can search or sort this table based on plasmid name, species, gene name, and more. Plasmid...
  9. Open Enzyme Collection

    Type
    Collection
    ...harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through...
  10. Viral Production

    Type
    Collection
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...
  11. Let There Be LITE Plasmids

    Type
    Blog Post
    ...MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors (TALEs...
  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...
Showing: 421 - 440 of 705 results