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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 710 results for: BASE;

Showing: 421 - 440 of 710 results

  1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...cells. A major bottleneck, however, to these IP-based assays is to find (or generate in-house) a pre-validated...develop a hassle-free, highly efficient Cas9 protein-based method for epitope tagging in mammalian stem cells...will have to design your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...cells are classified as pluripotent or multipotent, based on their ability to generate different cell lineages...Cell research. You can search or sort this table based on plasmid name, species, gene name, and more. Plasmid...

  6. Typing CRISPR Systems

    Type
    Blog Post
    ...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...

  7. Open Enzyme Collection

    Type
    Collection
    ...harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through...

  8. Viral Production

    Type
    Collection
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...

  10. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...

  12. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...results (Sinnett et al., 2017; Powers et al., 2019). Based on the work of the consortium, two gene therapy ...guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into the hippocampus...iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References: Amir ...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...point on the axis determined by the researcher, based on their controls. You can usually tell where this...Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to...

  14. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...

  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...environment. The “how many?” question lies at the base of all these questions as there is not particularly...for their buck - make them demand more services based on what they're spending. Increase the number of...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...off-target effect. The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of...express without the G. Because the G is only one base, the Zhang lab usually adds it when they order the...oligo from IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could...
Showing: 421 - 440 of 710 results