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  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...widespread use of FRET with fluorescent molecules, including fluorescent proteins, has led to the alternative...measuring the change in FRET efficiency, from which conclusions regarding the proximity of the two proteins can...

  3. 6 Tips for Grant Writing

    Type
    Blog Post
    ...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

  5. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

  6. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...

  7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...conducting literature reviews and having weekly journal clubs outside of work. Our scientific training was put...we had an idea, knew how to build it, but had no clue how to run a business. SynBio Axlr8r thought color-changing...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...

  10. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...introduce yourself and what you do. You might want to include your university and your advisor’s name for some... uploading your work to a preprint server (and include this information on the poster) so your visitors...

  11. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...

  13. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we...recombination using a repair template. These templates can include loxP sites to create conditional floxed alleles...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...are needed to optimize yield. You might want to include an extra wash step or use a different strain. For.... Look out for carbohydrates Certain strains, including HB101 and derivatives, release large amounts of...

  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

  20. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...One of the cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...
Showing: 421 - 440 of 793 results