We narrowed to 802 results for: Clu

1. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...frequently found in genetic testing for syndromes that include developmental delay, result in loss of function...

2. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have... field of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, ...

3. 6 Tips for Grant Writing

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   ...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...

4. Hot Plasmids and Viral Preps - January 2021

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   ...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ... infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and ...can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification...

6. Plasmids 101: Terminators and PolyA signals

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   ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

7. Tips for Titering Your Lentiviral Preps

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   ...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

8. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...

9. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ...conducting literature reviews and having weekly journal clubs outside of work. Our scientific training was put...we had an idea, knew how to build it, but had no clue how to run a business. SynBio Axlr8r thought color-changing...

10. Hot Plasmids: Summer 2025

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    ...AAV6 is reported to have broad tissue tropism, including skeletal and cardiac muscle, airway epithelial...be expressed Cre-dependently to allow tracing exclusively from genetically determined cells. This version...

11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...

12. Tips to Make the Most of a Scientific Conference

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    ...introduce yourself and what you do. You might want to include your university and your advisor’s name for some... uploading your work to a preprint server (and include this information on the poster) so your visitors...

13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, bowtie, UCSC...CrispyCrunch, be sure to check out the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput...

14. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ... maintaining stable lines of genetic mutants, including in situations where maintaining heterozygosity..., they have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource...

15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc 2, BioImageXD...to the properties of FPs themselves. These can include the potential to oligomerize, protein charge, and...

16. Plasmids 101: Knockout/Knock-In Plasmids

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    ...marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we...recombination using a repair template. These templates can include loxP sites to create conditional floxed alleles...

17. Addgene's Cancer Collection Pages Connect the Research Community

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    ...in the screenshot below). Currently, the page includes interactive maps for the Ras, mTor, and p53 pathways...from the research community, will soon expand to include several other important pathways. We invite feedback...

18. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...

19. Antibodies 101: Flow Compensation

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    ...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...

20. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very efficient introduction...using the Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: ...