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We narrowed to 957 results for: Cre

Showing: 421 - 440 of 957 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...isolated and screened. Depending on the marker, traps can be used as high-throughput screens to introduce...models. Why traps are useful Traditional genetic screens employ various on/off switches to measure the function...circumstances, trap methods may be the preferred screening option. Promoter traps Promoter trap systems ...101: Using Transposons in the Lab Plasmids 101: Screens vs. Selections Plasmids 101: Knockout /Knock-In...

  2. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries ...presence in the microbiology community. For our first concrete steps into this field, we’ve curated microbiology...

  3. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase....GFP-Cre.WPRE.SV40 AAVrg Recombinase James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg... viral prep AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) AAV-PHPeB Recombinase...

  4. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...cobalamin fusion to the riboswitch results in increased fluorescent signal. Prior to the development of...developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius and colleagues...Listen to the base editing podcast segment The increasing popularity of single base-editors has resulted...cytidine deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC...

  5. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat hippocampal...protein (CREB) in living cells. “Now we are making this really good,” Griesbeck said. “We know CREB is really...10.1038/nmeth.2773. (PubMed) Friedrich et al. Imaging CREB activation in living cells. J Biol Chem. 2010 Jul...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...photoconverts predominantly to the anionic form, causing increased fluorescence when excited with 488nm blue light...which allows PA-GFP to exhibit up to a 100-fold increase in fluorescence when illuminated with blue light...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't become...the absence of photoactivation resulting in an increased signal to noise ratio. PA-FPs with higher contrast...super resolution microscopy, PA-FPs have helped create several new imaging methods, including photoactivation-localization...

  7. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...roughly 200 cells. Increase the titer to 1013 resulted in a roughly 6-fold increase in expression [3, ...Figure 2]. Note how expression increases exponentially with an increase in titer. That means that once...to get more AAV in contact with the cell is to increase the AAV concentration. In this flowchart, you ...

  8. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...primers to create 200-300 bp sequencing products using PCR. A diagram of the product we created for the ...plasmid. However, lots of library DNA is needed for screening experiments, so the scale of the DNA prep is larger...rank the plasmids by abundance, scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz...wishes you luck in your future library use and screening, and if you have any tips, input, or stories of...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...of producing high protein animal feed. Due to increasing prices of methanol and advancements in other ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...addition to increased brightness, they also found that the signal to noise ratio can be increased from 2-fold...previous CRISPR-based loci visualization tools by increasing gRNA stability. First, they began with the Broccoli...and went through subsequent design iterations to increase stability and minimize misfolding and recombination...label two loci simultaneously, Ma and Pederson created another sgRNA containing the PP7 hairpin sequence...

  11. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ... lack of credit for small contributions. We strive to eliminate these two barriers, creating a community...with others than by oneself that prompted us to create Crowdfight.   Figure 1: Crowdfight is a ...collaboration between researcher and expert; 4) Credit all contributions.   Our volunteers are extremely...collaborations can be easily started and easily credited. And nothing stops these one-time favors from ...

  12. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...purple) and Gram negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio...charged iodine is added, it interacts with the CV+, creating a complex within the peptidoglycan layer, staining...peptidoglycan layer, trapping the large dye complex created by the interaction of CV+ and I- in the thick multilayered...positive and negative classification system is incredibly helpful in visualizing differentiations between...What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

  13. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...resulting in a decrease in luciferase activity. This experimental protocol can readily screen miRNAs for ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...pathways can be investigated in a high-throughput screen using luciferase activity as a measure of signaling...

  14. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...the potential for increased speed and efficiency through which knowledge is created and shared. Can you...platform. Expanding further beyond the US and increasing Addgene’s international presence would potentially...information, making it available to everyone, that creates more breakthrough and innovation. With any kind...science regulatory law. My role at Suffolk is to help create new programmatic content as well as help our students...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...that approach seems to create competition for your own spot. True! But you create a network of friends,... and simplify access to academic resources, we created STEAMid, for a nationwide database of  STEAM internships...with your question(s). This precautionary step increases the likelihood of acceptance to the internship... following graduation. While these suggestions increase your competitive edge, they alone, are no guarantee...

  16. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...sequence desired. This technology allows you to create a pair of designer proteins that will introduce... transfection or electroporation. All you do to create a protein that binds those 15 base pairs is take...genome editing tools allow you to do is greatly increase the efficiency of the genome editing process. ...for investigators without a lot of expertise to create zinc finger nucleases that will bind desired sequences...in terms of the target site. Also, any time you create double-strand breaks, there is a possibility of...

  17. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...address this need, the Khavari lab at Stanford created the RNA-protein interaction detection (RaPID) method...RaPID. Ramanathan et al. also tweaked BirA* to increase its rate of biotinylation. The improved enzyme...in neural progenitor cells. Knock-down of QKI decreased Zika viral RNA levels by 90%, suggesting that ...Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized by increased iron serum levels and early-onset cataracts, is...sequences and that loss of binding correlated with increased serum iron levels seen in HHCS patients. These...

  18. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...is a ~150 amino acid, single domain antibody. To create their RANbodies, Yamagata et al used previously... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...them. HRP RANbody staining results in a ≥10-fold increase in signal over staining with a primary/ labeled...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...also underwent editing through the NHEJ pathway. Created with BioRender.com. In later characterization...current tools, thus quieting some fears about the creation of “designer babies.” The differences in editing...disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not carry a given.... claim that CRISPR would still be helpful in increasing viable embryos, especially in the case of older...still be worthwhile for a given disease if it increased the percentage of disease allele-free embryos ...

  20. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence from unbound...CRISPR to label multiple loci in distinct colors. To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...was fused to a different fluorescent protein to create three distinct colors. The specificity of these...distinct hairpin sequences produces white light. Created with BioRender.com. With this rainbow of colors...combination, for example, with a far-red FP, would increase the available colors to 15! They also envision...
Showing: 421 - 440 of 957 results