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We narrowed to 1,145 results for: Gene

Showing: 421 - 440 of 1145 results

  1. How Addgene Creates Content

    Type
    Blog Post
    ...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...content collections is our Help Center, help.addgene.org, where you can easily search articles answering ...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...

  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... also modify other properties such as endogenous gene expression, which can lead to changes in normal ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... overview of the chemogenetics system. Why use chemogenetics Because chemogenetic receptors are activated...neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide Find viral preps...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it is...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose...most common target for chemogenetic manipulation are neurons where chemogenetic receptors can be used ...

  3. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you!...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... the mutagenesis reaction. Alternative approaches to site directed mutagenesis Point-mutagenesis is fairly...relevant in this age of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate...modifications.   Site-directed mutagenesis of Plasmids. A) Generation of mutagenized plasmids. PCR primers...also generates 4 bp overhangs. You can then use the Klenow fragment of DNA polymerase I to generate blunt...Series Use CRISPR to Generate Specific Genomic Mutations Resources on Addgene.org Browse Our Molecular...Laursen from Cornell University. Site directed mutagenesis is a highly versatile technique that can be used...analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution of key residues), and...

  5. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...work with fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. .... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...

  6. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... caters to optogenetics users and adjusts the lasers appropriately (tuned for optogenetics). These lasers...answer a research question. The flip side of the optogenetics coin is materials science-based. Light is delivered...optics is probably the least difficult portion of optogenetics (though it does require a significant amount...become go-to providers of materials needed for optogenetics. There are several important considerations ...with fabrication of the fiber optics needed for optogenetics. First, the length of the fiber optic for the...laser output), 5ms “on”, 50ms “off”.  A pulse generator such as the A.M.P.I Master-9 Pulse Stimulator,...manipulation of the laser power source itself. Pulse generation (TTL pulse) is a standard electronics protocol...

  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...insertions, deletions, and point mutagenesis to complex assembly of multiple genes. Primer design for each type...amplified, restriction digested or even a synthesized gene. PCR is the primary method to produce the linear...included in a single PCR, for example for subcloning genes from different templates. All PCRs are run as an... 5’ end of either primer. Subcloning - To move a gene from one vector to another simply amplify the vector...protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple... to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed by designing primers astride...using the same three step protocol:  Fragment generation - Any linear DNA fragments can be assembled in...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...-based genome engineering has revolutionized the gene editing field by making experimental workflows considerably... tools. As a consequence, a critical step in the gene editing workflow - analyzing the data - is often...likely to result in a functional KO of the targeted gene.   Analyze Complex Edits   Traditional Sanger sequencing-based...result in a functional Knockout (KO) of the targeted gene. The analysis can be sorted by any of the parameters...validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please contact us at [email protected]... more efficient than previous methods. Still, generating reliable results from CRISPR edit data requires...detect or analyze complex edits, such as those generated by multiplexed delivery of multiple sgRNAs. Since...

  9. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...provide an increased understanding of DNA binding and gene regulation. For additional information on CRISPR-mediated...technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed PMID: 26848818...interactions between genomic regions by enCh IP‐Seq." Genes to Cells22.6 (2017): 506-520. PubMed PMID: 28474362...the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 (2018): 318-325. PubMed PMID: 29480524...RNA-guided platform for sequence-specific control of gene expression." Cell 152.5 (2013): 1173-1183. PubMed...Fujita et al., 2018). Furthermore, the Fujii Lab generated a transgenic mouse line and retroviral expression...specific gRNA-dCas9 complex. When combined with next-generation sequencing, this method allows for the recovery...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...or negative selection). Next-generation sequencing (NGS) to identify genes of interest. Figure ...target genes for drug discovery or to understand the intricacies of molecular pathways. These screens have ...the targeting of hundreds to tens of thousands of genes with minimal effort. The versatility of Cas effectors.... (2022). AAV vectors: The Rubik’s cube of human gene therapy. Molecular Therapy, 30(12), 3515–3541. https... Adeno-associated virus as a delivery vector for gene therapy of human diseases. Signal Transduction and...Forward genetics screens are a valuable part of the molecular biology toolbox to identify new target ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression...

  11. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...a higher number of viral particles for efficient gene transfer. I figure it’s better to have a bit extra...derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...3) the transfer plasmid which encodes a transgene of interest that’s packaged into the virus. Two to five...

  12. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds ... one of the model organisms which I use to study gene regulation during developmental processes, CRISPR...Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker (Kim et al., 2014). Meanwhile... I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%...yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific...Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous...CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences https://doi.org...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400...deposited the associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more...with Addgene causes a paper to get cited more?   Articles with and without plasmids at Addgene To ...authors deposited with Addgene and those without plasmids deposited with Addgene. Together these journals...of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what... at Addgene – typically about four times as many as papers without plasmids deposited with Addgene (Figure...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...

  15. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...and explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous... activities at Addgene ...

  16. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

  17. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR ...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on...community. They did this by depositing them in Addgene’s repository. Other researchers, eager to try this... tools of their own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools...This growth is supported by new labs joining the Addgene CRISPR community, with approximately 100 new CRISPR...CRISPR depositors each year.    Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative... (B) Cumulative CRISPR plasmids deposited with Addgene by year. (C) Cumulative number of laboratories ...

  18. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science Video...change. Having been at Addgene for a month, I can say with certainty that Addgene is a company that cares...Sociology and Dance at Skidmore College. I was also Addgene’s Marketing Intern this summer! With the help of...made me intrigued by the marketing internship at Addgene. Although my interests are very clear, I had no...COVID was getting, I was hoping to walk into the Addgene office my first day and meet everyone but this ...Marketing Intern, I was tasked with continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed...project, I observed what it was like to work at Addgene. This is exemplified in my work interviewing fellow...

  19. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...antibodies via Addgene to maximize the reach and impact of your work. Please email deposit@addgene.org if you ...Antibodies and Grow From There Addgene’s antibody resource is funded by a generous grant from the NIH’s Brain...reagents, share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If...Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! ... your experiments. To promote reproducibility, Addgene will have unique Research Resource Identifiers ... preparations (Bradbury et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. ...

  20. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...media, you can find us on Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and...we will not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated...an official Addgene account. You can reach out to us directly via email at help@addgene.org, and we invite...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...mainstay of conversation in the scientific community. Addgene has been considering a gradual move off X as a ...to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent remarks and...posting, monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the ...
Showing: 421 - 440 of 1145 results