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We narrowed to 911 results for: IRE

Showing: 421 - 440 of 911 results

  1. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...contributed by guest blogger, Gary McDowell, executive director of Future of Research. On December 1st 2016, many...salaries - in some cases reversing these decisions entirely. At Future of Research, we had been collecting...are going ahead with raises anyway, including the entire faculty of the biochemistry department at the University...McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. He is interested in advocating...

  2. Working with Nuclear Receptors

    Type
    Blog Post
    ... some 400 molecules that have been shown to be required by one or more NRs for efficient and timely activation...Primary or core coregulators (those that interact directly with NRs) exist in steady-state complexes with...the new version of the NURSA website will feature direct links to listings of available plasmids for our...relationship with Addgene for the benefit of the entire research community. Thank you to our guest bloggers...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...reporters specially designed for the aims and requirements of in vivo studies. A particularly useful sensor...or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7...various AAV serotypes, researchers can precisely direct the calcium sensor to specific tissues or even ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

  4. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...heard from friends that Scientist positions often required a one-hour presentation about a prior research...manager or recruiter what presentation format they require. Frequently they will ask you to present about ...similar answer in a following interview. Expect to be tired once the interview is complete Industry interviews... interviewers might.  You’ll probably still be required to give a job talk for a remote interview. Fielding...

  5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...formation via NHEJ, precise modification with Homology Directed Repair (HDR), CRISPR activation or repression,... edits compared to DNA transfection.  Homology directed repair (HDR) with Nanoblades CRISPR-based HDR ...To utilize HDR, a donor template containing the desired sequence flank by homology arms must be delivered...with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm or...

  6. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...engineering for each loci, CRISPR/Cas systems only require changing the programmable guide RNA. Gene activation... “first generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest...activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic Activation... to other notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component...

  7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...that the artifactual PSCs commonly observed with direct stimulation are largely absent in the soCoChR-expressing...BBa_J23100) and expresses N-demethylases and other required enzymes, including a glutathione S-transferase... ΔguaB E. coli are missing an essential enzyme required for conversion of xanthine into guanine, and without...choice for FRET assays where a dark acceptor is desired due to its very low quantum efficiency. ShadowY...

  8. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...be dull and painful to sit through (imagine the entire audience playing Candy Crush or reading email).... interviews. The idea is to ask questions in a directed way to bring out these interesting stories and... happens, but get things back on track if the direction of the interaction is not interesting to the audience...politely but firmly get them to stop talking and redirect the conversation. The person you cut off might...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing... or just random mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’...Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature Communications...

  10. Pooled Library Amplifications

    Type
    Blog Post
    ...(Are the inserts of different sizes?), and the desired coverage for the library (How important is it that...  The most important factor to consider is your desired library coverage, since this will help decide what...determined experimentally. For larger libraries that require hundreds of nanograms of DNA, you will likely need...bacteria will be its own colony, and will not be in direct competition with other colonies containing smaller...

  11. PhD Applications After COVID

    Type
    Blog Post
    ...Potential PIs Even if you’re applying to a school that requires its students to do rotations in different labs...to introduce yourself to a few members of your desired department during the application process. Many...However, an online meeting with members of your desired program will allow for you to have the time and...is right for you outside of just the program requirements: by talking to current students about the work...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... steel wire into a backbone structure of a protein, based on its atomic coordinates. These wire models...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for BioMolecular Modeling and...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    ...also less accessible due to their expense and requirement for lab equipment. Plus they create plastic waste... discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits for different purposes...nucleic acids are released by dipping the cellulose directly into a PCR buffer, where DNA or RNA can be amplified...This method takes less than 30 minutes and only requires incubating the columns with 1M phosphoric acid...

  14. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...students published their work identifying new genes required for stem and progenitor cell maintenance in planarians...Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their work and become...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance in planarians...

  15. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...content-aware suggestion dropdown list will let you jump directly to the type of information you are looking for...straightforward search is now even easier with a direct link to the plasmid page at the bottom of the dropdown... dropdown list: The search bar will suggest direct links to other types of searches including depositing...search for “Melina Fan” and you’ll be able to go directly to a page to see the plasmids deposited by our...

  16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...description. You check off all the boxes - you have the desired degree, you have mastered the applications they...person. Similar reasoning may also apply to other acquired skills.” It is nice when other people point out... of a time you’ve lead your team in a defined direction in the past. Maybe there was a time you noticed...list all the skills that your current or past job requires day-to-day, and you’ll get a great snapshot of...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...screen for IV deliverable AAVs? Typically AAVs are directly injected into the brain, but this tends to limit... (figure 4f) of the tTA inducer virus, but the desired sparsity of labeling is achieved with lose doses...-color labeling of neurons, because it doesn’t require the generation of a transgenic mouse. 2. Cell-... gene vectors for neurons, no transgenic mouse required (see figure 5 and supplemental figure 8 in Chan...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...binding-dependent expression of the HIS3 gene, which is required for histidine manufacture and therefore growth...of interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset...at the same time in order to try and access the entire interactome of a set of proteins at once.  However...gain access to the nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin...

  19. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up and running...previous years, this event was held at Executive Director Joanne Kamen’s home- a testament to the familial...it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops prior... this saying is especially true. If your event requires a lot of set up or cleanup, ask for volunteers...

  20. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...ribosome. One resistance gene on your vector might require two different selection molecules! It all depends...option is to use a different method of selection entirely. Auxotrophic selection relies on the host organism...organism being unable to synthesize a nutrient required for growth. Before delivery of the vector, the deficient...
Showing: 421 - 440 of 911 results