We narrowed to 770 results for: LIS;

... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

...tool that you are looking for and check our curated list of biosensors too, as another laboratory may have...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

...up clear tasks for each member of the team to accomplish so that many elements of the project were able...Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member...

...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought...

... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

... PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for...PCR is that it can amplify DNA using only a short list of reagents and several heating and cooling steps...

...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...not map to the reference sequence. The resulting list of hits is then carefully reviewed. As mentioned...

...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ...

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...not better, than antibodies for all of the above listed molecular techniques, but they can also be expressed...

... to reflect upon before they attempt to make essentialist connections between biology and ancestral groups...the long and ugly history of reporting false essentialist links between biology and ancestry group as ...

...The tradeoffs between various cloning methods are listed in Table 2. For example,  chaining-based cloning...used in various chaining-based cloning methods are listed in Table 3. The goal of high throughput cloning...

...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ...

...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...

...Originally published Aug 30, 2018 and updated April 16, 2020. Figure 1: Comparison of SHERLOCK...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK...

...polling some Addgene colleagues, we’ve come up with a list of things to consider as you make this transition...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ...

...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version... See Supplementary Table 3 in Huang et al. for a list of PHP.B permissive and non-permissive mouse strains...