...PubMed Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific...

...Larry Gold, developed methods for selecting and amplifying RNA sequences that could bind very specifically...

...that this is just a review article and not your life’s work: so remember that done is better than perfect...

...requirements of the FLSA. In the University of California system, the postdoc union has negotiated a raise...

..., and feels privileged to be part of the vibrant life sciences research and commercialization community...

...genes that couple host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences...

... proteins in heterologous hosts. If you simply amplify a gene of interest from the human genome, for instance...

...Diego Biotech Lab 2018 Mexico e-nnovation Life Sciences 2020 India Sinapse Biotecnologia...

... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

...attention! They isolated DNA from the sample, amplified the CRISPR locus from the bacterial genome, and...

...vertebrate models such as mice, which are less prolific and more expensive per individual to maintain....

... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...

...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131 (DE3...

...expression mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, ...

...see if it should be amplified before you conduct your screen. If you need to amplify the library, please... Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read ...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to... 175,000 different gRNA-containing plasmids). Amplifying and Using the Library Once you receive your library...general steps to take in the screening process. Amplify the library (electroporation and maxiprep). If ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA...

... a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed. Regulation of DNA double-strand...

...saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template with a “sloppy” version...

...the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin...

...blot, and depending too much on normalization can amplify other errors in experimental design and gel quantification...