We narrowed to 570 results for: SHA

1. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ...align it with these new goals, which I’d like to share below. Common pitfalls in the transition (what I...

2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   ...multiple steps to avoid using multiple fragments that share homology in the same reaction. In these cases, the...

3. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   ...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources...

4. Viral Vectors 101: Biosensors

   Type

   Blog Post

   ...EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent Biosensors for Neurotransmission...

5. Viral Vectors 101: Types of viruses

   Type

   Blog Post

   ...Lentivirus Lentivirus is a type of retrovirus and shares many common features with gamma-retrovirus including...

6. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ...plasmids could be used by other labs to preserve and share their own collections. Why Recombinant Antibodies...

7. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   ...freeze-thaw-freeze cycle before, or otherwise been subject to mishandling?  Is the experiment you’re planning to run with...

8. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   ...less likely that this amino acid sequence will be shared across other proteins. Conversely, polyclonal antibodies...

9. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   ...SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the ...

10. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    ...perfected processes to curate the data our depositors share and enrich it with sequencing data; as a result,...

11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    .... If you have a great new plasmid you’d like to share with the scientific community, contact [email protected]...

12. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ...your notebook. This will make it easier for you to share plasmid information with colleagues, or explain ...

13. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ...):337-47. PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries...

14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    ..., certain that the impact of CRISPR scientific sharing will continue to grow, and we at Addgene appreciate...

15. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    ...30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases enable...

16. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    ... of the AsLOV2 domain allosterically alters the shape and binding ability of its nanobody partner, so ...

17. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    ...skillset and interests. As such, I want to be able to share more about this nontraditional career path for others...

18. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    ...just one reaction. Using other methods, such “BAC shaving” would require multiple steps. One potential pitfall...

19. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...It’s a simple solution. It would be silly not to share it as soon as possible. Addgene: How much interest...

20. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    ...post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of...