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We narrowed to 732 results for: SON

Showing: 421 - 440 of 732 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  2. Hot Plasmids - October 2020

    Type
    Blog Post
    ...and inhibitory synaptic proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal...library from Addgene New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources References...Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research...

  5. Tips for Technical Support Calls

    Type
    Blog Post
    ...! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet searches are done via...potentially faulty product. Please remember that the person at the other end of that email you’re writing is...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...and so should be handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element...

  7. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibodies recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). ...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation.      New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...20463753. PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...panelist or audience member from taking over. My own personal rule is that I do not let the panelists introduce...stop talking and redirect the conversation. The person you cut off might be miffed but the other 200 audience...

  11. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ...

  12. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...consider performing PEG precipitation. Instead of sonicating the cell pellet, I lyse the cells with this AAV... Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production...

  14. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...are interested in experiencing Cas9 up close and personal, visit the 3D Molecular Designs web site. Or, ...

  16. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Thankfully, this is a hot topic with many resources! Bondeson et al. (2022) reviews all the tags discussed here...choice!   References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR ...

  20. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified.     New from the viral service By Jason Nasse We regularly add new viral aliquots from our...
Showing: 421 - 440 of 732 results