We narrowed to 452 results for: SUP

1. Hot Plasmids: Spring 2025

   Type

   Blog Post

   ...HeLa cells were infected with 96 h lentiviral supernatant and selected with puromycin. Puromycin-resistant...

2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ...Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the Mouse and...

3. Generating Mouse Models Using CRISPR/Cas9

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   Blog Post

   ...of Acadia National Park. I was confused. It was supposed to be hard. I was mentally prepared to go through...

4. PiggyBac-ing Through the Genome Editing Field

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   Blog Post

   ...a greater number of integrations per cell and a super-piggyBacTM can be used to get an even greater number...

5. Immunocytochemistry

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   Protocol

   ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: January...

6. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   ..., which is conserved in many organisms. When supplemented with adenine analogs such as 2-fluoroadenine...

7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   ...using. Contact Addgene ([email protected]), or the supplier of your vector, to obtain this information.  Optimize...

8. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   ...transposons in bacteria sets the stage for combining the super powers of CRISPR and transposons to allow for the...

9. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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   Blog Post

   ...on developmental biology at the École Normale Supérieure de Lyon. Her love for educational science led...

10. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...

11. Antibodies 101: Antibody Engineering and Directed Evolution

    Type

    Blog Post

    ...means improving research. More specific antibodies support common research tools like western blots, ELISAs...

12. What is Polymerase Chain Reaction (PCR)

    Type

    Protocol

    ... reaction with both. MgCl 2 acts to supplement the amount supplied by the buffer stock, where it is known...

13. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    ...assembly can occur. It is quite extensive with a supplemental add-on kit and is compatible with E. coli and...

14. Pouring LB Agar Plates

    Type

    Protocol

    ... not supposed to be resistant to the antibiotic, (+) indicates that the tested strain is supposed to be...

15. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations...

16. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors...

17. Deep Dive: qPCR

    Type

    Blog Post

    ...tissue types, appropriate reference genes (and supporting data) are often documented in the literature....

18. Delivery Methods for Generating iPSCs

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    Blog Post

    ...insertion can also disrupt the expression of tumour suppressor genes and/or oncogenes, potentially leading to...

19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    ...the specific gRNA(s), and the repair template is supplied as a dsDNA fragment. Each round of editing takes...

20. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...vector production, the Rep and Cap regions are supplied in trans , via a different plasmid. In order to...