We narrowed to 447 results for: SUP

1. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   ..., which is conserved in many organisms. When supplemented with adenine analogs such as 2-fluoroadenine...

2. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   ...using. Contact Addgene ([email protected]), or the supplier of your vector, to obtain this information.  Optimize...

3. Plasmids 101: Using Transposons in the Lab

   Type

   Blog Post

   ...transposons in bacteria sets the stage for combining the super powers of CRISPR and transposons to allow for the...

4. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   ...on developmental biology at the École Normale Supérieure de Lyon. Her love for educational science led...

5. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...

6. Immunocytochemistry

   Type

   Protocol

   ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: January...

7. Plasmids 101: Modular Cloning Applications and Kits

   Type

   Blog Post

   ...assembly can occur. It is quite extensive with a supplemental add-on kit and is compatible with E. coli and...

8. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ... reaction with both. MgCl 2 acts to supplement the amount supplied by the buffer stock, where it is known...

9. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

   Type

   Blog Post

   ...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors...

10. Pouring LB Agar Plates

    Type

    Protocol

    ... not supposed to be resistant to the antibiotic, (+) indicates that the tested strain is supposed to be...

11. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations...

12. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    ...insertion can also disrupt the expression of tumour suppressor genes and/or oncogenes, potentially leading to...

13. Deep Dive: qPCR

    Type

    Blog Post

    ...tissue types, appropriate reference genes (and supporting data) are often documented in the literature....

14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    ...the specific gRNA(s), and the repair template is supplied as a dsDNA fragment. Each round of editing takes...

15. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...vector production, the Rep and Cap regions are supplied in trans , via a different plasmid. In order to...

16. 15 Hot Plasmids from 2017

    Type

    Blog Post

    ...set out to “rule” Molecular Biology. Under the supervision of Dr. Tan, a team of undergraduates created ...

17. Technical Design of a Western Blot

    Type

    Blog Post

    ...published antibodies, check the manuscript and supplementary figures for validation data, or follow the references...

18. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    ...antibodies are coming directly from tissue or supernatant, and you’re doing the purification and cross-...

19. Viral Vectors 101: Systemic Capsids

    Type

    Blog Post

    ...thank Viviana Gradinaru and Tim Shay for their support and input. Resources and references Recommended...

20. Protocol - How to Inoculate a Bacterial Culture

    Type

    Protocol

    ...plasmid. However, a liquid culture is capable of supporting a higher density of bacteria and is used to grow...