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We narrowed to 736 results for: Sis

Showing: 421 - 440 of 736 results
  1. Viral Vectors 101: Voltage Indicators

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    Blog Post
    ...Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on...
  2. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ...the cell pellet, I lyse the cells with this AAV lysis buffer and freeze/thaw the lystate four times in...-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol...
  3. Antibodies 101: Flow Compensation

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    Blog Post
    ...you can adjust compensation values later in your analysis, unlike voltages.  Figure 1A shows a single-color...always change your compensation values during the analysis portion of your experiment. So, as long as you...
  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one has to target...
  5. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ... a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to easily screen...thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element...
  6. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...
  7. DNA Purification Without a Kit

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    Blog Post
    ...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due...columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial...
  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...began engineering new versions of GFP through mutagenesis in order to improve its physical and biochemical...
  9. Plasmids 101: Stringent Regulation of Replication

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    Blog Post
    ...however, the protein integration host factor (IHF) assists with the process: it bends the DNA to redistribute...with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids may result in...
  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post
    ...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...
  11. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ... well received, understand that people can be resistant to change. Ask your peers or your superiors to...to explain their rationale when they’re resistant to new ideas. If their answer is “we’ve always done ...
Showing: 421 - 440 of 736 results