Skip to main content
Addgene
Showing: 421 - 440 of 729 results

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained virions from...can then remove the density gradient material by dialysis/buffer exchange and further concentrate the virions...

  4. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...you can adjust compensation values later in your analysis, unlike voltages.  Figure 1A shows a single-color...always change your compensation values during the analysis portion of your experiment. So, as long as you...

  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although we used... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...Dolan, A. E., Liao, M., & Ke, A. (2018). Structure basis for RNA-guided DNA degradation by Cascade and Cas3...

  8. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... easier, and more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies...& Platt, R. J. (2023). Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Nature, 622(7982...

  9. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one has to target...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to easily screen...thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element...

  11. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    ...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due...columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...began engineering new versions of GFP through mutagenesis in order to improve its physical and biochemical...

  17. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...

  18. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...environmental pollutants, drug discovery, and disease diagnosis. As biosensors, aptamers hold a few important...largely in vitro; Easy and reproducible commercial synthesis (those of us who have used antibodies for diagnostics...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...image shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense...
Showing: 421 - 440 of 729 results