Skip to main content

We narrowed to 731 results for: Sis;

Showing: 421 - 440 of 731 results
  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although we used... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...
  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable...
  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on...
  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one has to target...
  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to easily screen...thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element...
  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...
  7. DNA Purification Without a Kit

    Type
    Blog Post
    ...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due...columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial...
  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...began engineering new versions of GFP through mutagenesis in order to improve its physical and biochemical...
  9. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...alternative tRNAs Codon optimization With low cost DNA synthesis, one of the primary ways researchers solve the...created codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online...
  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...environmental pollutants, drug discovery, and disease diagnosis. As biosensors, aptamers hold a few important...largely in vitro; Easy and reproducible commercial synthesis (those of us who have used antibodies for diagnostics...
  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...
  12. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...
  13. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...however, the protein integration host factor (IHF) assists with the process: it bends the DNA to redistribute...with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids may result in...
  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...screening, followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov...scripts provided by the depositing lab for data analysis.  I'm often asked whether this step can be skipped...
  15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...
  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ... well received, understand that people can be resistant to change. Ask your peers or your superiors to...to explain their rationale when they’re resistant to new ideas. If their answer is “we’ve always done ...
Showing: 421 - 440 of 731 results