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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: Sis;

Showing: 421 - 440 of 738 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination of the transcript...: PMC102582. Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible to interpret...sequentially increasing fineness helps to maintain a consistent stream of light. Test your implant by connecting...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...Dolan, A. E., Liao, M., & Ke, A. (2018). Structure basis for RNA-guided DNA degradation by Cascade and Cas3...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... easier, and more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies...& Platt, R. J. (2023). Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Nature, 622(7982...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained virions from...can then remove the density gradient material by dialysis/buffer exchange and further concentrate the virions...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...the cell pellet, I lyse the cells with this AAV lysis buffer and freeze/thaw the lystate four times in...-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol...

  13. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...you can adjust compensation values later in your analysis, unlike voltages.  Figure 1A shows a single-color...always change your compensation values during the analysis portion of your experiment. So, as long as you...

  14. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although we used... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on...

  16. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one has to target...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to easily screen...thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element...

  18. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due...columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial...
Showing: 421 - 440 of 738 results