We narrowed to 501 results for: Trac;

1. Plasmids 101: Knockout/Knock-In Plasmids

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   ...endogenous promoter, you can use expression GFP to track cells participating in development or other physiopathological...

2. 3D Printing Meets CRISPR Cas9

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   ...These wire models were useful in that they not only traced the 3-D path of the protein’s backbone in three-dimensional...

3. Google Forums Round Up: First Impressions of NgAgo

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   ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

4. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ... is sedimentation equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers...

5. Experimenting with New Careers while in Grad School

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   ...an excellent resource that has gained a lot of traction with researchers. It is an excellent way to meet...

6. Which Fluorescent Protein Should I Use?

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   ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...

7. Plasmids 101: Secondary Nanobody Toolbox

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   ...but they can also be expressed in bacteria and extracted with common protein purification methods. Read...

8. Transferable Skills Guide: Identifying Your Transferable Skills

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   ... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...

9. Tips for arabidopsis transformation

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   ...the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your...

10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...generation of such resources in less genetically tractable organisms lags behind. BiFC can also be used to...

11. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...dire than Zimmer could have imagined. Does this detract from the quality of the book? It doesn’t need to...

12. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...expression for the data likelihood contains an intractable integral of the product of a Poisson and multivariate...

13. Droplet Digital PCR for AAV Quantitation

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    ...place.  When the PCR is complete, a droplet reader extracts the droplets from the plate and measures the fluorescence...

14. CRISPR 101: Making a Knock-In Cell Line

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    ...500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et...

15. Negative Can Be Positive: Open AAV Data with Addgene

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    ...providing DOIs, this information can be cited, tracked, and appropriately credited, broadening the knowledge...

16. An Introduction to Adenovirus

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    ... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events...

17. Plasmids 101: Protein tags

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    ... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...

18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...functions in the hope of developing drugs against intractable diseases. Currently, a number of labs are using...

19. Your Lentiviral Plasmid FAQs Answered

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    ...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

20. Transferable Skills Guide: External Collaborations

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    ...being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral...