We narrowed to 506 results for: Trac;

1. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   ...expression of lentiviral vectors make them an attractive option. However, safety requirements and expression...

2. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   ...is a physicist with a background in optics and a track record of developing new microscopy tools for biological...

3. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   ...endogenous promoter, you can use expression GFP to track cells participating in development or other physiopathological...

4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   ...anemone’s red colored tentacles resemble worms which attract fish for them to prey upon (Figure 1). Figure...

5. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

6. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   ... is sedimentation equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers...

7. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   ...an excellent resource that has gained a lot of traction with researchers. It is an excellent way to meet...

8. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...

9. Tips for arabidopsis transformation

   Type

   Blog Post

   ...the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your...

10. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...but they can also be expressed in bacteria and extracted with common protein purification methods. Read...

11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    ... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...generation of such resources in less genetically tractable organisms lags behind. BiFC can also be used to...

13. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...dire than Zimmer could have imagined. Does this detract from the quality of the book? It doesn’t need to...

14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    ...expression for the data likelihood contains an intractable integral of the product of a Poisson and multivariate...

15. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ...providing DOIs, this information can be cited, tracked, and appropriately credited, broadening the knowledge...

16. An Introduction to Adenovirus

    Type

    Blog Post

    ... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events...

17. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    ...500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et...

18. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...place.  When the PCR is complete, a droplet reader extracts the droplets from the plate and measures the fluorescence...

19. Plasmids 101: Protein tags

    Type

    Blog Post

    ... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...

20. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ...functions in the hope of developing drugs against intractable diseases. Currently, a number of labs are using...