...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

...through qualitative data that they are a long-term solution to reproducibility and cost-effectiveness for ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

...versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells... like the addition of a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence...

...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

...or licenses that could prohibit Addgene from distributing the plasmid, select “yes” from the drop-down...but can accept sequence files in any format. Distribution status for your plasmids: Hold for Publication...

...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

...enhanced response. Evolution of a new variant Maria and the team set up a directed evolution and screening ...

...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...

... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...

...explosion of software tools accompanying the CRISPR revolution. (See omictools for even more.) However, none...McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg...

... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...

...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...

...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...

...recombine DNA? There are many methods that have been utilized over the years to move around pieces of DNA. Oftentimes...in the DNA. In restriction cloning, scientists utilize specific restriction enzymes to cut dsDNA of interest...reducing the amount of time required for cloning. To utilize this approach, the fragment of DNA that you would...pairs adenine (A) and thymine (T). TOPO® cloning utilizes the Taq polymerase which naturally leaves a single...traditional restriction enzyme cloning. Scientists have utilized this approach to create compatible custom overhangs... 100kb) compared to other cloning methods that utilize bacteria where it becomes progressively more difficult...

...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine..... AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51...

...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in...

...tools for human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons...

... absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as ...elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9 fused to a biotinylation tag expressed on...