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  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ..., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

  3. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence...

  4. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...

  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...great overview of the development of CRISPR into a varied research toolbox, as well as additional resources...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

  9. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...into a vector, scientists may utilize one of a variety of cloning methods (restriction enzyme, ligation...research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid use...

  11. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

  12. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene...

  13. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  14. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...
Showing: 421 - 440 of 767 results