We narrowed to 776 results for: Vars;

1. Plasmids 101: What is a plasmid?

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   ...into a vector, scientists may utilize one of a variety of cloning methods (restriction enzyme, ligation...research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid use...

2. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   ...BioGRID ORCS resource will be adaptable to a wide variety of screen formats and contexts and continue to ...improve the accessibility of CRISPR screen data to a variety of users. Many thanks to our guest bloggers from...

3. Hot Plasmids and Viral Preps - July 2021

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   ...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced... Various Addgenies...

4. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   ...great overview of the development of CRISPR into a varied research toolbox, as well as additional resources...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

5. Fluorescent Proteins 101: Fluorescent Biosensors

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   ...biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence...

6. SunTag and Fluorescent Imaging

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   ... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...

7. Antibodies 101: Fc Effector Functions

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   ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

8. Plasmids 101: Gene, enhancer, and promoter trapping

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   ...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene...

9. New Viral Vectors - Fall 2024

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   ...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

10. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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    ...Do you prefer being a generalist? Working on a variety of projects and in different fields of research...Everyday was the same and I was bored. I wanted more variety and more power to influence the direction of my...

11. Management for Scientists: What Makes a Good Manager Anyway?

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    ...Every worker at every level does better with some variety in their job. Good relationships with clients and...mental space and dislike intrusions (this actually varies, a manager does best to identify the work style...

12. Advice for Choosing a Research Project

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    ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...

13. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... Various Addgenies...

14. Addgene Begins Distribution of Recombinant Antibodies

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    ...the community about how the antibodies work in various applications, enabling you to better plan your ...antibody, the target antigen, and performance in various applications are included in the full webpage. ...

15. RUBY-Red Siliques

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    ...plant cell culture), which is great for a wide variety of applications. But some techniques require you... RUBY plant. (Bottom panels) RUBY expression can vary from different transformation events likely due ...

16. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...improving the gRNA design process and rules for various applications is necessary going forward. The standardization...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

17. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ...fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused...Localization of antibody-combining sites within the variable portions of heavy and light chains. Proceedings...

18. Single-cell tracking of lineage and identity with CellTag

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    ...Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique ...

19. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    ... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification...

20. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...