We narrowed to 772 results for: Vars;
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TypeBlog Post... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...
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New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...great overview of the development of CRISPR into a varied research toolbox, as well as additional resources...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase... -
Plasmids 101: What is a plasmid?
TypeBlog Post...into a vector, scientists may utilize one of a variety of cloning methods (restriction enzyme, ligation...research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid use... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...BioGRID ORCS resource will be adaptable to a wide variety of screen formats and contexts and continue to ...improve the accessibility of CRISPR screen data to a variety of users. Many thanks to our guest bloggers from... -
New Viral Vectors - Fall 2024
TypeBlog Post...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)? AAV Variables that Matter... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...Every worker at every level does better with some variety in their job. Good relationships with clients and...mental space and dislike intrusions (this actually varies, a manager does best to identify the work style... -
Advice for Choosing a Research Project
TypeBlog Post...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...the community about how the antibodies work in various applications, enabling you to better plan your ...antibody, the target antigen, and performance in various applications are included in the full webpage. ... -
RUBY-Red Siliques
TypeBlog Post...plant cell culture), which is great for a wide variety of applications. But some techniques require you... RUBY plant. (Bottom panels) RUBY expression can vary from different transformation events likely due ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...improving the gRNA design process and rules for various applications is necessary going forward. The standardization...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... Various Addgenies... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique ... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post... the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...system could be used to deliver or measure new variables. The sky is really the limit for this emerging... -
Antibodies 101: Epitope Tags
TypeBlog Post... for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a variety of antibodies...