We narrowed to 772 results for: Vars;

1. SunTag and Fluorescent Imaging

   Type

   Blog Post

   ... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...

2. New Viral Vectors - Fall 2024

   Type

   Blog Post

   ...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

3. Antibodies 101: Fc Effector Functions

   Type

   Blog Post

   ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

4. Plasmids 101: Gene, enhancer, and promoter trapping

   Type

   Blog Post

   ...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene...

5. Management for Scientists: What Makes a Good Manager Anyway?

   Type

   Blog Post

   ...Every worker at every level does better with some variety in their job. Good relationships with clients and...mental space and dislike intrusions (this actually varies, a manager does best to identify the work style...

6. Advice for Choosing a Research Project

   Type

   Blog Post

   ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...

7. Addgene Begins Distribution of Recombinant Antibodies

   Type

   Blog Post

   ...the community about how the antibodies work in various applications, enabling you to better plan your ...antibody, the target antigen, and performance in various applications are included in the full webpage. ...

8. RUBY-Red Siliques

   Type

   Blog Post

   ...plant cell culture), which is great for a wide variety of applications. But some techniques require you... RUBY plant. (Bottom panels) RUBY expression can vary from different transformation events likely due ...

9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... Various Addgenies...

10. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    ...improving the gRNA design process and rules for various applications is necessary going forward. The standardization...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

11. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    ...fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused...Localization of antibody-combining sites within the variable portions of heavy and light chains. Proceedings...

12. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    ...Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique ...

13. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    ... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification...

14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

15. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    ... the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...system could be used to deliver or measure new variables. The sky is really the limit for this emerging...

16. Antibodies 101: Epitope Tags

    Type

    Blog Post

    ... for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a variety of antibodies...

17. Viral Vectors 101: Transductions

    Type

    Blog Post

    ... incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver nucleic...number of ready-to-use viral preps available for a variety of broadly applicable and popular viral vectors...

18. Don’t Wait To Launch Your Health Science Startup

    Type

    Blog Post

    ...management team). Sreyan is a research associate at Harvard Medical School and runs everything science (CSO... get through to a number of experts, including Harvard/MIT faculty, venture capital and angel investors...

19. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    ... start somewhere. I knew if I started with the Harvard Business Review or some Chartered Institute of ...further education may allow you to transition into various realms of business.  Thank you to our guest blogger...

20. Extracurricular Activities for a Strong Science Career Path

    Type

    Blog Post

    ...resource allows the undecided to learn about a variety of science professions based on their skills and...future regulations impact your work? What design variables need consideration? Enrich your knowledge and ...