We narrowed to 1,091 results for: addgene
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TypeBlog Post... who each are being awarded free plasmids form Addgene to accelerate their research. Read on to learn ...how these researchers plan to use plasmids from Addgene to advance their research on diseases of glycosylation...this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune...and other specific gene sequences available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl...neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors as well as...
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Developing Lab Management Software for Biology
TypeBlog Post...placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...proteins. The T-DOGGs and Cre-DOG are available from Addgene, and we’re eager to see how you use them in your...PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific...26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation of protein...Biol. 19 (2012), 117–121. PubMed PMID: 22157958. Addgene Resources Cre-lox Resource Guide Fluorescent Protein... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post... More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral Protocols...10.1007/978-3-319-07758-1_2 More resources on the Addgene blog Viral Vectors 101: Viral Applications Viral... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...resources by sharing plasmids Find plasmids at Addgene ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other... -
Antibodies 101: Affinity Tags
TypeBlog Post...-59259-301-1:99 Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols...having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...protocols Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each... -
Year of the Camelid: Antibody Style
TypeBlog Post...from addgene.org Plasmids encoding nanobodies at Addgene Sybody Generation Toolbox from Addgene ...to human populations around the world. Here at Addgene, we love camelids too, though not just for the ...10.1038/s41586-021-03676-z More resources on the Addgene blog Introduction to Antibodies Secondary Nanobody... -
Hot Plasmids - October 2022
TypeBlog Post...available! by: Ashley Waldron The majority of Addgene's more than 50 antibodies target neuroscience-related...protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant...love to hear from you! Fig. 3: Image of Addgene’s western blot for the myc-tagged protein GFP-smFP-myc...Anti-c-Myc [9E10] page for more details. Image credit: Addgene. Find anti-c-Myc antibody here! Evan, G... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...careers here Resources on Addgene.org Learn more about careers at Addgene ...Twitter @HansPacker. Additional Resources on the Addgene Blog Get tips on how to do your own informational... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...below and an Addgenie will help you find what you need. More Plasmid Resources from Addgene: Plasmids... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that... of the specific mutations. For these purposes Addgene has a large collection of yeast expression vectors...Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of common epitope ... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog Post... This is just the first of many milestones Addgene is poised to reach in the near future! We’re almost...April 8, 2021) Find these stats and more on the addgene.org homepage! You can track the number of plasmids... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process... the plasmid sequences). As with all things at Addgene, each part of this new process involves a significant...available as a partial sequence with the heading "Addgene Partial NGS Result." In some cases, there will ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find ...examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in our particular...over 6 years for 35 papers (data was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...viral vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual...hosted by individuals and by organizations like Addgene (a good example is our series of blog posts on ...Reproducibility at Protocols.io Additional Resources on the Addgene Blog FPbase: A new community-editable fluorescent... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...1 are available at Addgene. Table 1: List of pCXLE episomal plasmids, their Addgene ID numbers and the...Field Sign Up to Write for the Addgene Blog! Resources on Addgene.org Browse Our Stem Cell Collection... diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells have been ...reprogramming factors they encode Episomal Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL...PMCID: PMC2922531. Additional Resources on the Addgene Blog Stem Cell Models for Disease & Open Science... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene Read David Riglar's FOR Boston 2015 Blog Post... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...resources by sharing plasmids Find plasmids at Addgene ...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other... -
Grad School Advice Part 2: Building Community
TypeBlog Post...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog Post...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...