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We narrowed to 1,090 results for: addgene

Showing: 421 - 440 of 1090 results
  1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ...University Medical Center are now available at Addgene along with a description of their experimental ...The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6...Maddalo et al., 2014). These tools are available at Addgene and could be useful to labs wishing to create this...including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from...technology guides, FAQs, and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT ....1089/hum.2015.087 Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome...
  2. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ...adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies, our scientists...measuring viral titer and assessing purity. But Addgene is also conducting more rigorous quality control...plexWell technology, we can now perform NGS at Addgene to sequence all the DNA packaged inside the viral...AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation, limitations,...commonly performed for research-grade materials. Addgene is going the extra mile and pioneering the systematic... Press, pp 247–278 Additional resources on the Addgene blog Check out our Viral Vectors Featured Topic...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...
  3. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post
    ...yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make...possible. Connecting the research community - Addgene's cancer collection pages Scientists are making...latest resources? In response to this challenge, Addgene has launched an initiative to curate our collection...direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen...section below. Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing...Institute: Ras Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a ...Used in Cancer Lineage Tracing Resources on Addgene.org Visit our Cancer Collection Pages Deposit Your...
  4. DIY DNA Ladders from Penn State University

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    Blog Post
    ...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...
  5. We're Updating Our Hold for Publication Policy

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    Blog Post
    ...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...website, Help Center, or email us at deposit@addgene.org. ...
  6. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    Blog Post
    ...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...
  7. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    Blog Post
    ...talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell...blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid...designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list...sequences coming soon!  Additional Resources on the Addgene Blog How to verify your plasmid Check out our ...featured topic page Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular...
  8. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ...resources at Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other...as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile...
  9. Seeing Red: Simple GFP Photoconversion

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    Blog Post
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...
  10. Interview with Bart Newland, Addgene’s Newest Board Member

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    Blog Post
    ...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...
  11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post
    ...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...
  12. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post
    ...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...
  13. Hot Plasmids: Summer 2025

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    Blog Post
    ...editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping ...planning on lounging poolside or swimming laps, Addgene has pooled libraries for any summer project. While...  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort...effort to expand your gene delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why...projection neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses... viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ...
  14. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...needed to expand the HEK293 cells. Check out the Addgene AAV Production protocol for more details. Pro tips...because I don’t have enough AAV. Compared to the Addgene AAV Production protocol, I take a lazier approach... the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more details...PubMed PMID: 18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool ...
  15. Deck the Lab 2022!

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    Blog Post
    ...Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will ...or Instagram with #DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have...
  16. PEI Calculator for Planning AAV Packaging Transfections

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    Blog Post
    ...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...PubMed PMID: 18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs ...for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here...
  17. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

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    Blog Post
    ... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...
  18. Editor's Choice, July 2016

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    Blog Post
    ... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...to read all of our excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse...
Showing: 421 - 440 of 1090 results