We narrowed to 599 results for: arl

1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ... QC process. While this has worked well for the early sets of SARS-CoV-2 materials, it will not scale ...

2. How Addgene Creates Content

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   ...final check before publication.   Do research! Nearly everything we write entails a primary literature...

3. Plasmids 101: Screens vs. Selections

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   ...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

4. AAV Q&A with Tim Miles

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   ...Would you recommend any liver specific capsid, particularly one that can target the epithelial cells in ...

5. Hot Plasmids - February 2022

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   ...create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall...

6. Viral Vectors 101: Types of viruses

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   ...to specific cell types of interest. This is a particularly nice feature as organ-specific injections, or...

7. OCaMP: A New Calcium Indicator for Neural Imaging

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   ...Scientists have developed calcium indicators spanning nearly the entire visible spectrum (Figure 1). The GCaMP...

8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   ...researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...

9. Viral Vectors 101: The AAV Retrograde Serotype

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   ...these vectors can exhibit spurious expression, particularly at the injection site (Fischer, KB. 2019). The...

10. Antibodies 101: Monoclonal Antibodies

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    ...for your experiment Monoclonal antibodies are particularly useful when you want to ensure that the antibody...

11. A Novice's Perspective on Neuroscience - SfN2017

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    ... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...most abundant molecules in a cell, this system is clearly also robust enough to ensure high levels of gRNA...

13. CRISPR 101: Non-Homologous End Joining

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    ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...test was the perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR...

15. 3 Challenges in Plant Synthetic Biology

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    ...challenge associated with molecular plant parts, particularly for applied projects intended for commercialization...

16. Plasmids 101: Yeast Vectors

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    ...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast...

17. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    ...knock-out mice, but for various reasons it is not nearly as efficient in human stem cells. What genome editing...

18. Seven Tips for Using LinkedIn as a Scientist

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    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...

19. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...having difficulties in amplifying a template, particularly from a large genome, you can recheck your primer...

20. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression...