We narrowed to 571 results for: bli
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TypeBlog Post...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...
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Summer SciComm Series: Modes of Communication
TypeBlog Post...bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging ... -
Getting to Know Expansion Microscopy
TypeBlog Post...experiment or evaluating ExM images in a new publication. Why Expansion Microscopy? Traditional... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post... selected and ranked based on the previously established sgRNA rules and potential on- and off-target... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug... -
MXS Chaining
TypeBlog Post...high-throughput cloning strategies available for assembling these modular types of constructs, and different... -
Lentiviral Vector Uses and Overview
TypeBlog Post...McGarrity GJ, Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes.... -
CRISPR Plasmids - Drosophila
TypeCollection...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression... -
CRISPR Plasmids - Prime Edit
TypeCollection...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...have been used successfully in experiments and published in peer reviewed journals. If you need to generate... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They... -
QC Sequencing Technologies at Addgene
TypeBlog Post...efficiency through methods like automation. All assemblies from our pipeline are now run through an auto-align... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...our educational resources. From 2013–2023, we published 110 videos, which received over two million views... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This... -
Hot Plasmids - November 2023
TypeBlog Post...points appear as fluorescent bands in the fiber, resembling a “ticker tape” recording. The authors created...