We narrowed to 636 results for: erf

1. Networking and Inspiring Young Scientists at Freshers Fairs

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   Blog Post

   ...foundations upon which young scientists can build powerful networks and we encourage them them to keep up...

2. Sleeping Beauty Awakens for Genome Engineering

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   ... is one such system which has been designed to perform gene transfer in vertebrates. The system is comprised...

3. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ... history of code making and code breaking is wonderfully analogous to genetic circuits discussed at this...

4. Management for Scientists: What Makes a Good Manager Anyway?

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   Blog Post

   ... Sometimes this means you have to fire a good performer if they are enough of a jerk to make the rest ...

5. Advice for Choosing a Research Project

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   Blog Post

   ...pro in a field, but to be trained to think and perform like a scientist. Researchers should  pick a topic...

6. The Stingy Scientist: How the Baby Gel Box Was Born

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   ...country and we are quick to throw out chipped or imperfect equipment.  We can’t safely recycle these materials...

7. Plasmids 101: Antibiotic Resistance Genes

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   ...Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...

8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...elements Read about four considerations for the perfect AAV tool Learn about synthetic promoter AAVs for...

9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ... gRNA sequences. This allowed tracing of the performance of each gRNA multiple times within the same experiment...

10. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ...sequencing to determine serotype AAV packaging is not perfect and while we think of the AAV vector genomes as...

11. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    ...injection. How long post-injection did you wait until performing the experiment? What detection methods did you...

12. Summer SciComm: Science Storytelling

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    Blog Post

    ... drives a story forward - and this drive is so powerful that it can entice an audience to come along on...

13. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post

    ... about the antibody, the target antigen, and performance in various applications are included in the full...

14. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

    ... a strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter...

15. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ...IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie...

16. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ...GFP in imaging  While GFP has been an immensely powerful tool to study proteins in vivo, it does have some...

17. Antibodies 101: Epitope Tags

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    Blog Post

    ...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...

18. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    Blog Post

    ...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...

19. Using Addgene's New Viral Service for Your Research

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    Blog Post

    ... gene, replace it, modulate its expression, or perform a genome-wide screen, we’ve got viruses to help...

20. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

    ...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...