We narrowed to 997 results for: lars;

... target to specific subcellular regions, laser ablation can damage other cellular structures, and genetic...activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength...the flip of a switch. You may be familiar with popular light-sensitive ion channels used to control activities...Opto-RhoGEFs to study cell junction integrity in the vascular barrier of the endothelium, but they could be ...

...lymphocyte population distinct from cellular debris and other larger cells. Note, we don’t mind the warning... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...

...Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced...protein to study its function is one of the most popular applications of fluorescent proteins. These fusion...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...291.5512 (2001): 2408-2411. PubMed PMID: 11264536. Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...

...IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...sections of papers that have successfully used your particular antibodies and refer to the manufacturer’s recommendations...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...Performing Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https:...

...- mK+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Table 2 provides a quick reference for some of the popular strains, their genotypes, and their primary use...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The ...

...Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh where he... - 7:11 : Introduction - Ghanaian scientist's molecular biology needs and why Kwabena applied for the ...Ghana after his PhD and the need to de-mystify molecular biology. 25:51 - 30:56 : Further biology work ...current resources to develop microbiology and molecular biology research programs. 30:57 - 40:16 : Kwabena's...Microbiology Pages Find Synthetc Biology Plasmids Find Molecular Biology Resources ...

... (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...the epigenome may make certain processes, like cellular reprogramming of iPSCs, more efficient. In general...genes of previously uncharacterized enhancers. The popular TALEN-based LITE system, which uses light to regulate...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9... use. If you want to study the effects of one particular modification for which a targeted editor, like...

...remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor...Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying the bacterial cell ...cell cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete ...CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does not work well...

...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...for skill development include leadership of extracurricular organizations, working in student government... impacts of health services research. She is particularly interested in driving scientific and technological...

...bound to the intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...number of signaling cascades, inducing different cellular responses. One of the biggest advantages of such...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer (FRET), in...

... the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles ...new resources with the scientific community – particularly ones that help address ongoing problems, like...catalogues Literature searches utilizing Google Scholar alerts and the NCBI LitSuggest Tool Following the...CLASTR results by minimal number of markers and similarity score match. This tool’s algorithm can be adjusted...

...Caveats of SunTag Very large size: a 24x scaffold fully occupied with GFP has a molecular weight of 1,400 kDa...to amplify fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn ...time to get enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of...thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum et al. observed...

...Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express the insert...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes...specific mutations. For these purposes Addgene has a large collection of yeast expression vectors with various...studying YGOI's function in worms. Some of the more popular studies involve using a reporter vector to tag ... Addgene: Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of...

...and are ready for a larger commitment, start your own blog. Plan to write regularly and, when you are ready...self-interested reasons, and, given that science is largely funded by tax-payers, there are reasons to feel...Twitter can be a good way to follow news about particular subjects or find out what others are reading....Natural Sciences and the Morehead Planetarium. Similar things are likely happening in your community, ...

...incomplete or nonexistent. In the 21st century, many molecular biologists didn’t know the complete sequences ... This algorithm enables one of SnapGene’s most popular aspects - its ability to annotate a raw plasmid...source of common features was our collection of popular plasmid sequences. These plasmids contain features...beginning or end of the feature. With the increasing popularity of gene synthesis, many researchers now use codon-optimized...Scientist at SnapGene, and also a Professor of Molecular Genetics and Cell Biology at the University of...

..., SeeDB2 is not powerful enough for large brain samples. For large brain samples, we recommend pretreatment... to achieve highest expression levels. Clearing large tissues - Fluorescent proteins are very stable in...our SeeDB Resources website. We update the site regularly and hope Tetbow can help you in your research....assistant professor at Kyushu University. He has a particular interest in the development of sensory systems...

...production can be a frustrating process that requires a large investment of time and resources for labs that don...Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository are used to... phenotype of interest. Optogenetic tools for similarly controlling neuronal activity with light will ... soon. We’re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent...AAVs are getting to the right cells using your particular delivery technique and conditions. Finally, we...

...cloned into a plasmid of interest. You can use similar processes to add overhangs to your insert of interest...primer design and the PCR process itself are very similar to those outlined in our restriction cloning post...based cloning     The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18 bases of the ORF, including the stop...

... immune cells. Dendritic cells (DCs) are particularly popular whole cell therapeutics because they can...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...through these various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is...production, and CTLA4 and CD protein blocking. Other molecular targets under research include TIM-3, LAG-3, STAT...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

...screening experiments, so the scale of the DNA prep is larger. And since the library contains many plasmids, ...amplification protocol, but the overall workflow is similar for most libraries. Here are the key points you... For libraries where the plasmids are all very similar sizes, like a gRNA library, this is less of a concern... hope you’ll agree with me that they look very similar to each other - that’s exactly what we want! At...your amplification. I hope this post has helped clarify some of the important considerations when using...