We narrowed to 775 results for: mos

...meeting with no idea why you are there. Make the most of everyone’s time by considering the level of meeting...to meet monthly, but we found our meetings took almost two hours and we felt out of touch. By moving to...

...Updated Mar 26, 2020. At their most basic level, CRISPR/Cas9 genome editing systems use a non-specific...crRNAs from pre-crRNAs derived from CRISPR arrays. In most systems used for CRISPR-mediated genome editing,...

...pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity light...number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter ...

...fluorescent proteins in common use (particularly not the most recently published ones), they can be a good starting...proteins the green and red proteins are generally the most detectable and so should be used to tag your least...

...identical, to their defined recognition sequence. Most enzyme manufacturers make High Fidelity versions... circular DNA takes on several conformations the most abundant being: supercoiled, relaxed and nicked....

...goals. These first hires will set the tone and atmosphere that future hires will look up to." She also ... "Running Your Lab" booklet. What has been the most surprising thing about becoming a PI? Tom Ellis ...

...available to the research community.    Validation For most cloning projects, the next step would be to validate...digest of the PCR products prior to cloning, which in most cases left the functional light chain intact, the...

.... While some developers had science backgrounds, most did not. It was my job to translate complex lab ...front-end and back-end coding frameworks to use. I mostly sit and listen to stay informed on the details,...

...research practices In an attempt to present only the most novel or significant findings, and combined with...really appreciate how few details are provided in most published papers. The precise timing, order, or ...

...integrated into the host genome upon viral transduction. Most transfer plasmids available from Addgene were derived...can be. Plasmids that encode SIN γ-retrovirus lack most of the U3 region of the 3’ LTR and are primarily...

...promoters Chemically regulated promoters are among the most common inducible promoters. The positive inducible... agents to allow promoter activation. One of the most commonly used prokaryotic promoters is the negative...

...nuclear envelope, which occurs during mitosis. The most well-known retrovirus is HIV. Lentiviruses are ... Gene Therapy with AAV Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...

...Overall, these approaches have two main drawbacks: 1) Most rely on transfection of more than one vector to ...lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments, a single vector ...

...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...on its own or be integrated within a bacterial chromosome and allows the donor bacterium to make a small...

...phenotypes that are hard to study. To overcome this, most scientists use mutant animals that are genetically...proteins in living cells. Until recently, the enzymes most frequently used for PL were APEX2 and BioID. APEX2...

...the light microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging...Since I started my PhD, I have been convinced that most microscopy techniques can be replicated with commercially...

...educational resources. Filters We identified the most useful kinds of information per resource category... details that make our new search engine unique. Most importantly, with this new implementation, we laid...

...intrigued by the tRNA processing machinery present in almost every living organism. Precursor, or pre-tRNAs ...levels of gRNA expression. Since tRNAs are among the most abundant molecules in a cell, this system is clearly...

...system you are really confident about working for most genes and individual targeting modules. Those two... following in mind: These libraries are pooled. Most of these screens that can be done with the libraries...

... a homologous template provided by the sister chromosome. As cells take those two ends and put them back... a technology that was difficult for all but the most experienced users and puts it in the hands of pretty...