...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection... to be easily concentrated. Viral RNA is then amplified isothermally, and detected through a toehold switch...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....modifying the sequence of the toehold switch. For example, Green has also developed toehold switches to detect...

...stable host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a...processes. The food processing industry, for instance, employs psychrophilic enzymes for milk processing, fruit...Additionally, the high activity of these enzymes implies that even a minute quantity can significantly reduce...

...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...redefine the value I could bring to potential employers. Over time, I discovered that I not only enjoyed...motivations, strengths, and learning styles. For example, during my time as a lab scientist, I worked with...foster a cohesive, productive environment. For example, in my current role managing a cross-functional...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

...endless ways and everyone finds their own. Here, I simply outline what works for me, and I hope that it is...experiments can be found in each folder. This can be a simple .txt document. Record the name, date, and location... work on multiple projects in a given day. For example, instead of naming a file “output” name the file...track of your exact changes. Alternatively, you can simply make a note in your methods section about the edit...result was, so try not to do this. These are the simple rules that I try to follow in my own research. ...

... Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation of endogenous... assemble a synthetic transcription activation complex consisting of multiple distinct effector domains...with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang lab for...sam.genome-engineering.org/. Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, ...

...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...detecting homodimerization tendency. This assay employs a peptide derived from cytochrome p450, CytERM,...will be problem-free. In figure 1 we provide an example of the OSER assay with a number of fluorescent ...organisms that are engineered can be shared to simplify the comparison by others. Together, we can build...

... that actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...system contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of...interest.   CcdB resistant E. coli strains complete the system But ccdB alone isn’t the only key to...protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed...

...strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

...where one of the X chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic...translate those signals into cellular responses. For example, GPCRs and their downstream G protein partners ...with an emphasis on oligomerization, signalling complex assembly, and localization to the nucleus,” says...at McGill University.  These protein-receptor complexes consist of many parts that work together on the...gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental delay, and...

...problem.  Step Three: Decide on a strategy and implement it.  Step Four: Assess how effective your strategy...the cause. To test the most likely cause, you implement an experiment designed to fix the problem. Then...modifications, and ultimately decided on the changes to implement. As with any skill, there will always be room ...Know your strengths! Research experience presents ample opportunities to practice problem solving skills...problem solving skills can help convince a future employer that you are well equipped to work through challenges...

...proteins within a system. This can be pretty complicated given that a single cell has tens of thousands...strategy a single protein can be isolated from a complex mix using affinity chromatography against an amino...metal ions are immobilized on a bead or resin and a sample containing the protein of interest flowed through...interest and the metal ions.      Fig. 1: A sample containing a polyhistidine tagged protein of interest...tags are also recommended if you need a very pure sample. Because binding strength is proportional to the...

... channels and pumps in developmental biology.  Examples of some ion-channel and ion-pump encoding genes...genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43...also modulating bioelectric states in vivo. For example, the Levin lab from Tufts University has used Arch...sensitive fluorescent voltage indicator with near-complete desensitization in continuous light. ArcLight ...extremely exciting emerging field, with many implications for regenerative medicine, evolution of body...

... about how the system operates and functions” “Complete lack of information on number of postdocs” “Postdocs...comments are well correlated with published data. For example, in 2010, less than 15% of postdocs had achieved...beginning to address these issues independently; for example, Harvard’s Therapeutics Graduate Program has a ...postdocs in academia, industry and other areas of employment? How many graduates and postdocs – and from what...organisations that represent postdocs and institutes that employ them, the diversity of trainee backgrounds, publishing...

... the mutation or replacing the gene. A notable example is the treatment of Severe Combined Immune Deficiency...normally functioning T cell repertoire (1). In this example, it was possible to transduce the T-cells outside...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies against ...: 14566358.  2. Hirsch, M. L., et al. "Self-complementary AAV mediates gene targeting and enhances endonuclease...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability... a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence flank by homology...Nanoblades which carries a gRNA, Cas9, and a HDR donor template DNA can be generated by incubating Nanoblade particles...helps form DNA and retroviral-particle or VLPs complexes. The team found that "all-in-one" Nanoblades facilitated...facilitated HDR with either a ssDNA or dsDNA donor template. In vivo editing of mouse zygotes and mice with...

...coordination to more complex situations such as learning and memory or emotional states, for example anxiety or ...and cause variability in the testing group. For example, if an experimenter picks up a mouse from the home...kHz, produced from fluorescent light bulbs, for example) that are outside the humans’ audible spectrum.... have preferences for specific cell types. For example, AAV1 and AAV9 serotypes have strong neuronal tropism...

...task, action, and results. While this method seems simple, I found it hard to remember to describe all four.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...interview. Expect to be tired once the interview is complete Industry interviews are long! Mine lasted 4.5 ...interviewer and then thank them once the interview is complete. Remember, your interviewers took time out of ...a question. Maybe you ask for questions after completing each section of your talk. Some video conferencing...

...process you would like to monitor? Neurons, for example, have unusually fast calcium dynamics, whereas ... is most sensitive to your expected range. For example, calcium in the ER lumen reaches the sub-millimolar...single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength measurements...crucial advantage: tissue-specific targeting. By employing various AAV serotypes, researchers can precisely...Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo. Neuroscience Research...

...sequence can completely disrupt expression of the transcribed ncRNA (Jiang et al. 2014).  Simple knock-out...transcription of the ncRNA (Hazan and Bester 2021). For example, inserting a polyadenylation signal downstream ...interactions between ncRNAs and adjacent genes are complex and not yet fully understood, so it is important...variants may also be useful for targeting ncRNAs. For example, the Cas12a variant can cleave single-stranded ...

... don't do anything. Viruses are complicated and they perform complex biological tasks, both of which ...virus-based experiment at one point or another. After amplifying a viral vector, collecting virus, and transducing...replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize...vector using the harvested viral solution as a PCR template. Regardless of the type of virus you’re working...