We narrowed to 563 results for: pac

1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   ...: Use a PAM-out configuration Optimize your spacing D10A: nick sites separated by 37-68 bp H840A: ...

2. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   ...Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated ...

3. Some Like it Hot: Thermostable GeoCas9

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   ... AA PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal...

4. Tips for Improving Your Next Manuscript

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   ... then the data produced isn’t likely to have an impact because it will be difficult or (more likely) impossible...

5. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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   ...these small tokens of thanks for the incredible impact our depositors have had on Addgene and the scientific...

6. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ... the academic community has clearly made a huge impact on this field. Advances in genome engineering ...

7. Management for Scientists: What Makes a Good Manager Anyway?

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   ...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...

8. Choosing Your Perfect Empty Backbone

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   ...however, remember that you will need accessory packaging vectors to generate the virus. Bacterial vectors...

9. Choosing a Good Mentor for Scientists

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   ... and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

10. New Viral Vectors - Winter 2025

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    Blog Post

    ...our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible...

11. Summer SciComm: Science Storytelling

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    ...explaining what new questions are next or how this will impact future research or policy.  It is worth mentioning...

12. Intro to Our Summer SciComm Series!

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    ...and intent to the messaging - while also creating space for people outside of the primary audience to engage...

13. Addgene Begins Distribution of Recombinant Antibodies

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    ...antibodies via Addgene to maximize the reach and impact of your work. Please email [email protected] ...

14. Hot Plasmids and Viral Preps - September 2021

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    ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ...problem in the context of IHC, but the problem can impact other techniques like Western blots or immunocytochemistry...

16. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    ...level of intimacy that creates the magic of a safe space. No matter why, now is a great time to find a few...

17. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post

    ...protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between...

18. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...Addgene account for Tech Transfer Offices. They’re packed with tech transfer resources (summaries, electronic...

19. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post

    ...self-promoting—No one is going to tell you to pick up the pace Constant worry about grants and renewals What is...

20. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...feature is advantageous if you’re working with small capacity viral vectors such as AAV. Base removal RNA-guided...