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  2. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...of AAV plasmids will have technical or legal restrictions that make them ineligible for use in this service...consideration will have a banner on their material page. Interested scientists can expand the banner to select their...

  3. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...double-stranded DNA break at the genomic loci of interest and on either side of the cargo (ex: fluorescent...the double-stranded break in the genomic loci of interest. As the universal sgRNA is not predicted to target...

  4. Understanding GPCR Signaling Using TRUPATH

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    Blog Post
    ...activated in response to a ligand binding a GPCR of interest. How does TRUPATH work? The TRUPATH collection...transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2...

  5. Fostering communication in remote collaborations

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    Blog Post
    ...between recurring meetings, you should share interesting results or ask questions to your collaborators...microscopes and allowed me to investigate my protein of interest in a French clinical cohort, neither of which ...

  8. Some Like it Hot: Thermostable GeoCas9

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    Blog Post
    ...discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can...CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check out this post...

  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...

  10. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post
    ...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you...

  11. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various... downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer...

  13. Meet the New Addgene Board Members!

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    Blog Post
    ... development was something Stegmeier has been interested in for a long time. “At an early age, I was fascinated...reagent, it’s an annotated reagent.” He’s also interested to see Addgene expand the sharing platform to...

  16. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    Blog Post
    ...a graduate student at Kyoto University. He is interested in developing genetic tools for neuronal circuit...professor at Kyushu University. He has a particular interest in the development of sensory systems focusing...

  18. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post
    ...have in common? You can do quality science on interesting topics Having strong non-science skills is important...mentors, to discuss your strengths, weaknesses, interests and passions is another great way to shed light...

  19. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...getting researchers to the experiments they’re interested in faster by producing, titering, and testing...neuronal activity while studying your phenotype of interest. Optogenetic tools for similarly controlling neuronal...

  20. What the HEK?

    Type
    Blog Post
    ...with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral particles...be a great option! Especially if your research interests include receptor biology, ion channels, or involve...
Showing: 421 - 440 of 755 results