Plasmids 101: Mammalian Vectors
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... for cells that have taken up the plasmid in the same way. Selection methods, described below, are typically...
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Fluorescent Proteins 101: Luciferases
TypeBlog Post...Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred from a luminescence... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...NGG, NGAN, NGNG, NGAG, and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies to map the... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...resistance gene followed by the target site (the same sequence you’d like to mutate in the genome) and... -
Viral Vectors 101: Biosensors
TypeBlog Post...monitoring of different factors, in different or the same cellular location, all at once! Small molecules ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...naturally, rarely are the optimization parameters the same for all three components, meaning that the final... -
Viral Vectors 101: Types of viruses
TypeBlog Post...low immunogenicity, meaning it does not induce the same high immune response as retroviruses. This virus... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found that they could... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...While both lentiviruses and γ-retroviruses use the same gene products for packaging (Gag, Pol, and Env),... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...designed to make you sick…well, you feel it. But the same features that make these familiar viruses such a... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...evolution to create APEX2. They have now applied the same directed evolution technique to the biotin ligase... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...suggests a change to a slide, think about whether the same principle applies to other slides in your deck. ... -
Plasmids 101: Inducible Promoters
TypeBlog Post...work in prokaryotic cells and typically only in the same or closely related species from which they were ... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...While walking around the exhibition area, I got a sampling of the broad variety of tools in neuroscience ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...systems typically express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...characterized and the system allows you to add this same antibody to any protein to get really beautiful ...