We narrowed to 610 results for: sam

1. Advice for Scientists Starting a Lab

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   ...useless.” How do you run your lab differently or the same as your adviser did? Tom Ellis merges the influence...

2. Plasmids 101: Mammalian Vectors

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   ... for cells that have taken up the plasmid in the same way. Selection methods, described below, are typically...

3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...resistance gene followed by the target site (the same sequence you’d like to mutate in the genome) and...

4. Luminescent Imaging with Nano-lanterns

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   ...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies to map the...

6. Top Requested Lentivirus and AAV of 2016

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   ...systems typically express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate...

7. Communicating Your Science With Help From ComSciCon

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   ...suggests a change to a slide, think about whether the same principle applies to other slides in your deck.  ...

8. Plasmids 101: Inducible Promoters

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   ...work in prokaryotic cells and typically only in the same or closely related species from which they were ...

9. A Novice's Perspective on Neuroscience - SfN2017

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   ...While walking around the exhibition area, I got a sampling of the broad variety of tools in neuroscience ...

10. Building and Validating Recombinant Antibodies: A Case Study

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    ...detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found that they could...

11. 15 Years of Addgene: The Top 15 Plasmids

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    ...expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...evolution to create APEX2. They have now applied the same directed evolution technique to the biotin ligase...

13. Fluorescent Proteins 101: Luciferases

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    ...Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred from a luminescence...

14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...While both lentiviruses and γ-retroviruses use the same gene products for packaging (Gag, Pol, and Env),...

15. Viral Vectors 101: Biosensors

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    ...monitoring of different factors, in different or the same cellular location, all at once! Small molecules ...

16. GCE4All: Making Genetic Code Expansion Accessible

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    ...naturally, rarely are the optimization parameters the same for all three components, meaning that the final...

17. Viral Vectors 101: Types of viruses

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    ...low immunogenicity, meaning it does not induce the same high immune response as retroviruses. This virus...

18. Viral Vectors 101: Viruses as Biological Tools

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    ...designed to make you sick…well, you feel it. But the same features that make these familiar viruses such a...

19. Sharing is Caring: But How to Distribute Open Hardware?

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    ...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...

20. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...NGG, NGAN, NGNG, NGAG, and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9...