We narrowed to 538 results for: shi

... grant application? Are you familiar with the publishing or patent application processes? How about facilitating...

... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

...the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted...

...thanks to our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has...

... plasmids from this paper at Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent...

... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

... inserted or deleted, can result in an in-frame shift of mCherry and thus expression. As a proof of concept...

...blue light which revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead...

...protein (EBFP2) is expressed in an unregulated fashion and thus a marker for transfection. The other (...

...alongside your input control. Elute the DNA after washing and then incubate with Proteinase K to reverse ...

...frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently published a set of ...

...-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, turning expression...

... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...

...’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures, tons of ...

...consequently about a two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can...

...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

...desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the...

...photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains on either side...

... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

...does not depend on excitation power in a linear fashion. This implies that reducing the power 2-fold does...