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We narrowed to 846 results for: tat

Showing: 421 - 440 of 846 results

  1. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...is limited by definition, the kinome is a well-annotated collection of genes that can provide a useful ...useful for imaging or gene expression assays necessitating the screening of individual plasmids separately... gRNAs that score as hits in pooled screens, facilitating more rapid follow-up. We hope the biological...

  2. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...Also in June, the G8 Science Ministers issued a statement after a meeting in London on the global science...available to others got a nine percent bump in citations for their trouble, even when all kinds of other... as much about open access as it does about the state of peer review. 8)     That same issue of Science...

  3. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...eyestalk and transform them into a pulsating display imitating a moving caterpillar. The worm controls the snail...discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective..."Tongue replacement in a marine fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." ...

  4. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...has led us to develop a searchable and sortable datatable that contains validated gRNA sequences. Validated...of the validated gRNA table You can access the datatable here. Current table columns are target gene, species... interested in. You can use the carats in the datatable headers to sort alphabetically or in ascending...

  5. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...but many throughout the world are affected by devastating diseases that affect far fewer people and therefore...provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi - Spinal muscular...Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated with the University of...

  6. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...for scientific titles generated from the United States Standard Occupational Classification System  and...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...

  7. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...plasmid sharing during COVID-19 Realizing this limitation, we worked closely with the legal partners and...As infection rates were increasing in the United States, and many universities and Technology Transfer ...Proteins, and plasmids from several labs at Florida State University became openly available. Find plasmids...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...its gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this ..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its...

  9. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...Office of Postdoctoral Affairs at UNC, called her presentation “Disequilibrium, Disillusion and The Postdoc...pretty much said it all.  She cited the oft quoted statistic that only 15% of current postdocs will be academic...Biology at Harvard Medical School, titled her presentation “How to Train Your PI”.   She focused her talk...

  10. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes... Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,...Years of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? ...

  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2...resulting in a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio...These plasmids are available as a collection, facilitating screening across the transducerome, to gain ...

  12. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...techniques offer researchers different advantages and limitations.  The four main factors in which chemogenetics... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to... once can result in several hours of neuronal excitation or inhibition. This sustained activation period...

  13. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...you communicate your science, whether it’s in presentations, manuscripts, or social media. If you want ...usually ok for informal settings like lab meeting presentations. You can download larger images without the ...means the images are probably better suited for presentations and digital articles where it’s easier to give...

  14. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. ...fusion protein while it is still bound to the glutathione resin, eliminating the need for an extra clean...

  15. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ... making plasmid maps, but can also analyze and annotate any DNA sequence. SnapGene Viewer: The free SnapGene...donation-based plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained...analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates...

  16. An Addgene Summer Internship

    Type
    Blog Post
    ...balance and were content. As a worker, there are expectations whether or not you work in the lab. My first...capstone projects during the Summer Intern Poster Presentation.  As someone who had limited experience ..., I worked on my capstone project report and presentation, during the final week at Addgene. This report...

  17. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...another email notifying me of my acceptance. I was ecstatic! I was going to become a serious scientific wordsmith...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique... D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression in Pseudomonas...

  18. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...research is improving lives worldwide and the presentation made it clear how important it is for us to ...say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles! We also...early on Saturday morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the ...

  19. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...wild-type version of YGOI to rescue a mutated strain or a mutated version of YGOI to test the different...different phenotypes of the specific mutations. For these purposes Addgene has a large collection of yeast expression...
Showing: 421 - 440 of 846 results