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We narrowed to 800 results for: tec

Showing: 421 - 440 of 800 results

  1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ... school when she was hired full time as a Lab Technician. “I was so tired of being in school so I just... is not true at all. Racheal Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

  2. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...powerful development in neural tracing. Using these technologies in combination with molecular tools (e.g., optogenetic...

  3. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...of our mouse modeling blog series, we covered techniques that can be used to introduce genetic modifications...conditional knockin or knockout mice. While these techniques are each useful for introducing one modification...) and using PCR with the appropriate primers to detect your targeting vector or gene of interest. 2. Breed...

  4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...there were unusual patterns of DNA methylation detected in a human disease: cancer (Feinberg & Vogelstein...the original methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization...repeat and scFv–TET1 catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...described above, and not any particular cloning technique. Though rarer, you can find MoClo that relies ...assembled into many combinations. Even with current technologies, it can be extremely hard to predict how a single... if you want to learn more about this cloning technique! Many thanks to Rob Hurt for his help on this ...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field... obstacles for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges...taken advantage of what our current delivery technologies are suited for. For example, lipid nanoparticle...

  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...very easy — our tech transfer team will communicate with your organization’s technology transfer office... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...biological tasks, both of which are factors in the technical challenges they can present. Many researchers ...hail Marys that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors... quantification as measured with branched-DNA technology." Journal of clinical microbiology 37.6 (1999...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...predicted off-target sites, Cpf1 does not mediate detectable off-target cleavage. Most gRNAs directed low-...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic FLEx switch will express BFP in the... Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the presence of Cre, GFP expression is... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...degradosome, which contains enzymes that cut RNA not protected by secondary structure. It is thought that poly... tail is important for stability of the mRNA, protection from degradation, and is integral to the nuclear...Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts Resources...

  12. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already have...Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is growing. Some post... a position open up. Other companies, such as Genentech, will not. The point is that each company has ...

  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...the AAV production process. Either SYBR green technology or a TaqMan primer/probe set can be used and ...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...

  14. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... on and so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either...chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains a primer...Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. ...

  15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...plasmid, using the fragment exchange (FX) cloning technique which uses Type IIS restriction sites (Geertsma...protein of interest. The Seeger lab used the ELISA technique described in their protocol because it is less...protein is added and after a few washes, binding is detected using a colorimetric reaction. After the ELISA...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...adeno-associated virus vectors with sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15...addgene.org  AAV Titration by qPCR Using SYBR Green TechnologyAAV Titration by ddPCRAAV Purification by Iodixanol...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... where rhodopsin functions as both the voltage detector and fluorescent signaler. The pro of this system...several targeting domains. The quencher and voltage detector in this system was dipicrylamine, a lipophilic...high-speed, bright, red fluorescent voltage sensor to detect neural activity. Sci Rep 9, 15878 (2019). doi: ...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Advantages of the agarose cloning method The agarose technique should be useful to scientists who wish to rapidly... from a population of transfected clones. The technique is applicable to a wide variety of cell lines,...physiological properties. An advantage of this technique is the potentially large number of single clones...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology...discovery through the creation of innovative technologies. To date his group has develop several popular...catalytically active Cas9 nuclease." Nature biotechnology (2015). PubMed PMID: 26436575. PubMed Central...

  20. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes...cheaper, faster, and easier than prior editing techniques. More and more CRISPR tools are being published...how you will evaluate the outcome: Will you be detecting insertions/deletions using a mismatch repair assay...
Showing: 421 - 440 of 800 results