We narrowed to 653 results for: EPO

1. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

2. Lab Automation at Addgene

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   ...With our ever growing rate of plasmid deposits (over 100 deposits per month!), manual transformations were...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV ...expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result in cells where...

4. Hot Plasmids and Viral Preps - March 2021

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   ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...recently contributed a set of plasmids to the repository that will help epigenetic researchers custom ...

5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   ...scientists who were potential Addgene requestors and depositors. China is in one of the top 10 countries in number...was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking work in... labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine...

6. Gaining Leadership Skills Volunteering at a Professional Organization

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   ...As the Chair of the Advocacy Committee, I had to report to the Board of Directors and liaise with the Executive...accordingly. For example, the quarterly and annual reports I had to write on behalf of the Advocacy Committee...to overcome the personal frustration and submit reports in the format requested which in the long run was...

7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-... protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1...

8. Magnetic Control of Proteins: More than a Dream

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   ...magnetic fields (Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire .... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...

9. Tips for Using BLAST to Verify Plasmids

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   ...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries in NCBI...NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids!...

10. MXS Chaining

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    Blog Post

    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...