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We narrowed to 641 results for: ato

Showing: 221 - 230 of 641 results
  1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...effort to track down every last one of those gene regulatory proteins and the places in the genome where they... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...
  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...
  3. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...about what steps are most likely to fail. Our laboratory manager would definitely be interested to see...this case, the physical considerations of the laboratory make object composition a smarter choice than...
  4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...(Shaner et al, 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere...CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting the results of the...proteins. Fusions of CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing...
  5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...
  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...ClonTracer Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis...Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology...
  7. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...
  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...
  9. 6 Tips for Grant Writing

    Type
    Blog Post
    ...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...
Showing: 221 - 230 of 641 results