...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...

...preparation of the fiber optics is probably the least difficult portion of optogenetics (though it does...polishing. Efficiency of the implant should be at least 85% of the initial laser output. The same considerations...University.  His interests include the role of learning and memory in opioid addiction and science communication...

...account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook... into executing a successful business that the learning curve is pretty steep. But we can rely on one ... about this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun...

... are nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins...ladder on your gel and keep an eye on it and the leading line (dye front) visible in the sample buffer. ...stripping and reprobing. Just make sure to go from least to most abundant protein if you’re stripping, since...

... Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check...the addition of post-translational modifications Learn more molecular biology in our Plasmids 101 eBook...

...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting...fruitful. After the conference Bring back what you’ve learned from the poster session to your lab. Perhaps some...scientific communication, and scientific networking Learn how to set up and informational interview and Expand...

...pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your... repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid...Barcode Watermelon Library 5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX...

...FPs to highlight various subcellular structures. Please see Figure 1 for commonly used markers for major...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging ...

...continue to to build and develop this new resource! Please leave us your thoughts in the comments section below...Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer...

...fantastic model organism for many reasons, not the least of which is ease of transformation. There are many...finding true transformants more difficult. Don’t leave your plants on selective plates for too long: even...recovered from the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won...

...commonly result in a complete loss of function while nuclease-deactivated Cas9 (dCas9) can be tethered to a ...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive ...about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries ...

... in the primary literature, and as a result can leave scientists under-informed with respect to how different...the year, those that meet our minimum standards (clear and detailed methods and image descriptions, a minimum...computer files. If you asked your team members or colleagues: How much unpublished AAV data do you have sitting...

...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...Third-generation plasmids can be packaged with either system. Please note that the generation used to package the virus...Addgene Blog: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus...

...this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab...to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember...career center. Alternatively, it might even be a colleague. A mentor does not necessarily need to be someone...

...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...zebrafish zygote transforms into an organism with a clearly established body plan including head, eyes, and... tracked in whole brains of swimming, actively learning larvae, providing unprecedented views into cognitive...

...subsequently changed fields and my cohort of friends and colleagues had moved on as well, so I knew very few people...people, it was certainly an issue worth addressing. Clear expectations have been established for future participants... after our initial pilot, and look to take some leaps. Binning Singletons is its own organization, working...

...much trial and error to learn the scripting language, but our path forward was clear: to build one process...assembly algorithms meant that we already had a clear idea of the necessary steps to perform:    ...

... variety of expression systems. Keep reading to learn more about the Gateway cloning method and its advantages...conditions, the attL and attR sites can recombine, leading to the excision of the phage from the bacterial...Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony...

...contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different...nucleus, if the cell dies from injury, this protein leaks out into the cytoplasm, migrates out of the cell...About Cancer and Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study ...

...CRISPR 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled.... & Liu, D. R. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors....

...for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens... target cells. Poor tissue culture practices can lead to low titers and poor transductions. Cell lines...

..., using up valuable resources in the cell thus leading to slowed growth. Additionally, some protein products...well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists ...your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of...

...a first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an ..., would help improve split vector strategies and lead to greater and more consistent transgene expression...

...very high titers (Zingg et al., 2017), which can lead to cells in far away brain regions expressing your...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, ... result in overexpression of your gene and could lead to toxicity or trouble interpreting your results...

...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close...suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes,...can be used up quickly by abundant transcripts, leading to falsely lowered levels for less abundant transcripts...

...episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein...plasmids covered in this segment Hot Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes...

...me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th ...Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences🎵On the...

...this series, we’ll highlight one tip that we’ve learned in the lab that will hopefully make your life a...be able to locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab...

...If you have any questions on ordering plasmids, please feel free to email [email protected]. We’ll be happy...regulations are complex and may change suddenly. Please email [email protected] for the most-up-to-date ...