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  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

  3. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...

  10. MXS Chaining

    Type
    Blog Post
    ...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...restriction enzyme cloning, were more prohibitive at the time. With these limitations no longer an issue, bacterial...your life Rather than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing...

  14. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... of bait and prey against each other at the same time in order to try and access the entire interactome...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  15. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

  16. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...notebooks in the ELN, without having to add at the time of deposit, makes it far more likely that deposits...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond through...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...

  21. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

  22. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

  24. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

  26. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

  28. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak, Rising τ, and Decay τ of the three sensors... a snapshot of interacting proteins at a certain time and genomic location. TurboID improved on other ...

  29. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

  30. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...
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