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Showing: 661 - 690 of 836 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...cells used and the readout, this could be a major limitation. An alternative to the full genome CRISPR screen...PhD student. In particular, one slide of the presentation made a lasting impression on me. The slide showed...

  2. Typing CRISPR Systems

    Type
    Blog Post
    ...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...

  3. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...being able to specify and correct single-base mutations without introducing DSBs at all. Base editing ...are smaller and less likely to cause off-target mutations than those derived from naturally occurring cytidine...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...lane 1 is‌ lane 8. Common ways to keep membrane orientation straight are to mark the upper left-hand corner... Ensuring wash is happening under sufficient agitation (rocking) Add Tween-20 to wash buffer (can...

  8. 2022 Depositor Week

    Type
    Blog Post
    ...  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...

  13. CRISPR Cheat Sheet

    Type
    Blog Post
    ...cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult. CRISPR...

  16. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ... year, @BordeauxFoSho takes you through our interpretation of Buddy the Elf's journey from the North Pole...

  17. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR ...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    ... convenience. After an order is placed, we’ll facilitate the necessary MTA, then start production and ...

  19. Editor's Choice, July 2016

    Type
    Blog Post
    ...for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As...

  23. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...formatted and published as a report with a DOI for citation. If you’re considering requesting an Addgene antibody...

  25. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...Dallas was the first to be infected in the United States while caring for a patient, despite wearing protective...

  27. What's New in CRISPR - May 2019

    Type
    Blog Post
    .... Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of...
Showing: 661 - 690 of 836 results