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We narrowed to 1,025 results for: URE

Showing: 661 - 690 of 1025 results

  1. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ..., analyzed your data, and made your tables and figures. Now it's time to write. Sometimes, it's hard to...Linux.  Mendeley and Zotero have a lot of the same features, but this chart highlights the differences between...Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student...blog: Tips for writing scientific manuscripts: literature searching, reading, and organizing Tips for writing...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...course (read our blog post on it and to see old pictures of the lab!). By 2016, we wanted to be taken seriously...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety training...project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge...yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil microbiome...

  4. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...participants could share their notes together. Figure 1: Can you draw Spongebob from these instructions...visuals, or asking questions using the polling feature or the chat box. One key thing to remember regardless...getting to know the app or virtual platform and making sure you stay connected. If possible, test out the technology...connection,” says Abitua about her workshop. “We made sure that we had a backup plan in case someone’s internet...

  5. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in...differences in cell wall composition. Cell wall structure Gram positive bacteria, or monoderms, have a single...the gram negative bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA was fully unleashed by the Gao Lab’s work... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

  7. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...and with a group who all strive for a common big-picture goal. It is worth doing some some serious pre-work...position. Am I choosing the right mentor? Make sure your advisors will be good role models and teach... career path?  If you are considering this, make sure to choose an advisor that supports both paths – ...details and many more suggestions on planning for future success by finding and joining your dream lab, ...

  8. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image...potential. I often thought that performing a cell culture experiment myself would take less time than the...independence, while others required more guidance and structure. I quickly learned that my approach needed to ...approaches—one detail-focused and the other big-picture oriented. Their misalignment was slowing progress...

  9. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ... conference via social media. The podcast also features an interview with Dr. Stephani Page, the founder...about a week and then unfollow those who do not capture your interest during that week.” You can also find... Media as a Scientist: A Very Quick Guide" from Nature Jobs Blog While Twitter is a popular platform for...pointers on how to use LinkedIn to research potential future employers and to arrange informational interviews...

  10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...distribution and interactions of proteins within these structures. Enzyme-catalyzed proximity labeling (PL) uses...limitations of APEX2 and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and ..., the enzymes function across a much broader temperature range than BioID, which only functions optimally... the SH3 domain collection here! Teyra et al. Structure 2017. Pubmed PMID: 28890361. ...

  11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...pandemic. Though we’ve confirmed the expected features and sequences in each plasmid, none of these constructs...will give your research more visibility.    Figure 1: Illustration of SARS-CoV-2 from Philippa Steinberg... plasmids to confirm they contain the expected features and are free of mutation.  Once the samples arrive...scientists to confirm the plasmids contain all expected features and meet our QC standards. If any concerns are...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...can affect reported titer by either method so to ensure equal animal dosing, we advise that users keep ...into codelivery efficiency at different doses. See Figure 4 in this paper for more information.   Q6 & Q7...Brain potency for each individual is reported in Figure 4B (see left for image and link for paper.)    ...and smaller CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area...

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...preceded by GA or followed by TC. As shown in the figure below, a Dam methylase recognition site (underlined... diagnostics, we suggest double checking to make sure your results will not be affected by methylation... has the XbaI site blocked by methylation), the figure below illustrates how sequence visualization software...will be blocked by Dam or Dcm methylation, you can ensure this site remains unblocked by first cloning your...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...are required for NHEJ repair regardless of end structure, and dictate the major events of the pathway: ...polymerases (Pol μ and Pol λ), nucleases (Artemis), and structure-specific end cleaning enzymes (Aprataxin, Tdp2... obligatorily introduce indels. Given the end structure of the Cas9 DSB (blunt or near-blunt ends without...graduate student interested in determining how the structure of broken ends impacts how they are repaired. ...

  15. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...engineering technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous... “GM” by demonstrating the current benefits and future potential of the technology to the public in an...enjoyed exploring the outdoors and experiencing nature first-hand. He studied biology at Virginia Tech...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...for vector propagation. This post, along with a future companion post on mammalian vectors, will catch... you up on the core replication and resistance features of yeast vectors and explain how they differ from...eukaryotes and thus contain complex internal cell structures similar to those of plants and animals. Unlike...Saccharomyces cerevisiae; however, we’ve also noted some features required for the replication of fission yeast,...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...now-common business practices, like accepting e-signatures and designing a website users could easily navigate...grow accessibility alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013...

  18. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...before me had been assigned a structure paper with two single-panel figures, one of which was a cartoon ...molecular biology class. A Cell paper. With six figures. Averaging ten panels each. (For this story, it...notebook, opened it, found my outline, read over Figure 4, took a deep breath, and, re-oriented, continued...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...fluorescent in situ hybridization (FISH) and chromosome capture suffer from low resolution and can’t be used in...using all the colors of the CRISPRainbow. Figure 1: CRISPRainbow was used to track the localization...image represents the composite of fluorescent measurements from red, blue, and green channels overlaid ...with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...contain the desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682...all of the players becomes ever more important to ensure that you choose the most appropriate method for...

  21. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...indirectly, with each protein of interest.    Figure 1: The direct approach (left) versus the indirect...best to use the direct method when possible, or ensure that the primary antibodies are different species...

  22. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...produced Polyclonal antibodies are a heterogeneous mixture of many antibodies that recognize the same protein...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...

  23. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...and the electroporated cells re-adhered to the culture plate within 4 hr of treatment. In some primary...super-resolution light microscopy, comes from his recent Nature Communications article “SRpHi ratiometric pH biosensors...to each transcription factor. They then induced cultures of these transformants with various concentrations...predicts that this level of control will enable future researchers to better simulate physiological gene...

  24. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...context. While native purification preserves RNA structure and naturally occurring complexes present in the...which for RaPID is an RNA motif of interest. See figure 1 below for details on the key components of RaPID...in breast cancer patients. By digging into the literature and analyzing data from The Cancer Genome Atlas...–protein interaction detection in living cells. Nature Methods, 15, 207-212. PubMed PMID: 29400715. PubMed...

  25. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...possibly giving you a way to connect with them in the future. Some profiles have a blue Follow button. For less...LinkedIn page. This can include news, inclusion, culture and connections. Start your interview research ...and “plus skills” areas. Include a professional picture. It doesn’t have to be a headshot in a suit, but.... Try looking a bit over your shoulder for the picture. It tends to look like a police photo if you look...

  26. Working with Nuclear Receptors

    Type
    Blog Post
    ...CARM1 methyltransferase. This attendant signaling feature afforded by coactivator complexes suggests that...the NURSA website, the new version of which will feature a simplified user interface, expanded numbers of...associated with identifying plasmids in the research literature and acquiring them in a timely manner. Addgene...accordingly, the new version of the NURSA website will feature direct links to listings of available plasmids ...

  27. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ..., 2013). Two important features of this early system are its regulatable nature and its ability to multiplex...occurred. This powerful negative selection system ensures high editing efficiency; the only non-edited cells... pTargetF and pCas plasmids can be subsequently cured from the bacteria. Although developed in E. coli...Resources on the Addgene Blog Check out our CRISPR Featured Topic Page Learn more about Cpf1 Find out about...

  28. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...the impacts of neural circuits on behavior. Figure 1: An overview of the chemogenetics system. ...characterize the compound, or compounds, you choose to ensure reliable results (Goutaudier et al., 2019). Using...Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors...designed Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038...

  29. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...efficiently and accurately in a “single pot.’   Figure 1: Single-Pot Assembly: a) Graphic depiction of... sensor modules targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender...quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic showing...

  30. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify Protein Protein...that give the strongest biotinylation signal, as measured via Western blot, and that result in proper localization...these two bait proteins stop interacting. This feature is particularly useful for in-depth characterization... of spatiotemporally defined protein complexes. Nature communications. PubMed PMID: 28585547. PubMed Central...
Showing: 661 - 690 of 1025 results