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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 818 results for: cell

Showing: 661 - 690 of 818 results

  9. AAV Packaged on Request

    Type
    Collection
    ... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...

  11. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

  13. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in ...sdAbs over conventional antibodies include:  Excellent tissue penetration, thanks to their small size...

  18. Arf GTPase Family

    Type
    Collection
    ...concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249–1271. PMID: 31084567 (Link opens...

  19. TALEN Guide

    Type
    Collection
    .... Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter...

  21. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...-activated cell sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded...actively dividing cell types, whereas gamma-retroviruses can only infect mitotically active cell types. This...Using Packaging Cell Lines Gamma-retroviral vectors can be packaged using helper-free cell lines (Figure...-retroviral packaging cell line developed by Garry Nolan at Stanford. These cell lines contain gag-pol...and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for the infection of mammalian cells). Using...make stable cell lines, VSV-G is often put under an inducible promoter. Depending on the cell type, there... — T and B cell transduction Murine Leukemia Virus (MuLV) and HIV glycoprotein — CD4+ cell transduction...

  22. Plan Your Experiment

    Type
    Guide
    ...difficult-to-transfect cells. You can direct expression to specific cell types by using cell-specific promoters...human embryonic kidney 293 (HEK293) cells. For more difficult cell types, you may need to look into other...are good choices for more difficult cell types like primary cells. For CRISPR delivery, lentiviral and...deliver to target cells through microinjection or electroporation. The target cells translate the mRNA...complexes directly into your target cells. In this case, your target cells are not transcribing DNA to mRNA...possible genotypes within the resulting cell population. Some cells may remain wild type due to either (1...modifications . Cell Population The first step of validation is determining the type of cell population you...

  24. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...

  25. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...
Showing: 661 - 690 of 818 results