We narrowed to 1,054 results for: STI

..., B-B’, and C-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...

...application. These controls will allow you to distinguish real results from background noise or nonspecific...positive and negative cells. This can be achieved by adjusting your voltage using your single color controls ...

...advice for using Twitter, this post provides suggestions for using LinkedIn and Facebook. For each of ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...

..., but they do include HEPN nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

... development, organs of interest, such as the intestines, can be dissected at various times throughout... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. J. ...

... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...

...commercial antibody against them. There’s also a question about how specific those interactions are. This... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...

...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and a mutated version of the ...

...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...

...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

...sensory input from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory... demonstrate pain response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration...

...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

... in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, we used primary...the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins...

.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

...proteins interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with...

...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...

...impacts the expression level of the transgene. In existing strains of mice, the transgene can be maintained...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...

...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

...used to expand already-existing sets. Note that this tool relies on a stochastic search algorithm rather...

... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated...

...six different genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet...the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half life of 84 hours in...

...modifying enzyme) to pair with it can be queried by testing any number in the Level 2 assembly. These pathways...scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, synthetic ...

...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice...

...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... edit. If possible, consider following these suggestions: Edit the PAM along with your intended edit....

...sample protein is retained better, antibodies will stick permanently to a membrane that has been dried, no...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...

...enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently, FRET...

...cross-linked, DNA is digested into smaller pieces. Sticky ends created are then ligated together to create...interactions opens the door for answering more questions about chromatin conformations. The Xu lab presented...

...proteins, it’s important to note that despite their distinction by name, chromoproteins are actually a subset...wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to ...

...prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...

...transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your cells, wait...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

...limited to restriction enzymes - although many labs still love using them! With so many technologies currently...an ordered set of scarless ligations following digestion with Type II restriction enzymes. All of these...

... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways... you use them for! A well-developed biological question and experimental system are absolutely necessary...

...easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements in order...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...

...PMCID: PMC5915492. Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet...

...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...subclass. Once you know the answer to these two questions, refer to this table to find the right nanobody...