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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 724 results for: ACE

Showing: 441 - 460 of 724 results

  1. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ..., their makeup (in terms of nationality, gender, race, etc), or their career outcomes. Some of the more...their mentorship with the same critical eye they place on others within their labs - as a sort of over-...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental...inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80 under different promoters and create...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

  5. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ... presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves...plasma membrane and can be detected through a cell surface label. The viral vector also encodes for eGFP, ...

  7. Typing CRISPR Systems

    Type
    Blog Post
    ...function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy: class to type to.... However, knowing the basic diversity and the surface-level function of each type could lead you to new...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    ...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ... simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose, ...

  13. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...Addgene gave to me: the PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR...

  20. Deck the Lab 2022!

    Type
    Blog Post
    ...It's just unfortunate that we had no choice but to place it next to our lab trash (which coincidentally matches...
Showing: 441 - 460 of 724 results