We narrowed to 591 results for: ARL

1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   Blog Post

   ...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   ...promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression...

3. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations...

4. dTAG - You're it!

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   Blog Post

   ...proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   ...and quantitative analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found ...

6. Seven Tips for Using LinkedIn as a Scientist

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   Blog Post

   ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...

7. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...having difficulties in amplifying a template, particularly from a large genome, you can recheck your primer...

8. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...have a few tricks to simplify the process. First, nearly all of our Sanger sequencing is done from glycerol...

9. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   Blog Post

   ...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...

10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

12. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    ...that the goal and the impact of the project are clearly stated and align with the description of the RFA...

13. Hot Plasmids February 2024

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    Blog Post

    ...Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases) and minimal toxicity, including...

14. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ... aims and requirements of in vivo studies. A particularly useful sensor for neuroscientists is CaMPARI...

15. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post

    ...rules the best way to design GGA overhang sets, particularly for high-complexity reactions? The researchers...

16. Viral Vectors 101: Virus Safety

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    Blog Post

    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...

17. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

18. Corralling and Cornering the Goldilocks Antibody

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    Blog Post

    ...from the background noise. If the sample images similarly to the negative control, that informs us that ...

19. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...