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  1. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...required a one-hour presentation about a prior research project. In total, the interviews lasted from ...prepared with questions to ask. With a quick Google search for “questions to ask an interviewer,” you can ... they will ask you to present about your prior research. This is a chance to highlight your prior accomplishments...deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science...
  2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post
    ...removing the wild-type TadA from the complex the researchers developed a second set: The ABE8.x-d version...3.2x higher for positions A3-A4 and A8-A10. The researchers selected 8 of the tested ABE8 constructs (ABE8.8... base editing.  Try ABE8 base editors for your research! Base editing in primary human cells  Cell therapy... of ABE8 for primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells...
  3. Viral Vectors 101: Chemogenetics

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    Blog Post
    ...Find viral preps for your research Find chemogenetics plasmids for your research ...Jendryka et al., 2019), they have resulted in a search for alternative ligands, such as C21, CLZ, Perlapine...Sternson S (2019) Ultrapotent chemogenetics for research and potential clinical applications. Science 6436...
  4. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post
    ...(Bradbury & Plückthun, 2015a, b). Therefore, researchers from over 100 scientific institutions have proposed...scientists can now improve genetically-encoded research tools, it is shocking that the plasmids and sequences... (2012) Raise standards for preclinical cancer research. Nature 483:531–533 . https://doi.org/10.1038/...Reproducibility: Standardize antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a...
  5. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post
    ...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...generally contains the same sections as a primary research article, it’s important to understand that presenting...is more! Posters should not look like a primary research article condensed into one big page. Your sections...Michigan State University. The idea is that the main research finding is written across the center of the poster...
  6. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post
    ...Laboratory. Are you a science student or early career researcher looking to break into science communication? ...non-scientists in an unusual setting: Discuss your latest research in a local pub at a Pint of Science event. Or ...gives training in media skills, writing about your research and residential communication. If science journalism...'d like to directly involve the public in your research projects, you can set up a citizen science project...
  7. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...
  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...fluorescent tool with magnets would enable new research and applications that don’t yet exist, but it ... this was even possible. There is a variety of research on whether and how organisms respond to magnetic... non-biological conditions. So, they decided to search for a magnetic response among other proteins that...some hypotheses. They are excited to see other researchers from a range of fields, like quantum chemistry...
  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post
    ...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...
  10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...with any scientific research, choice of a technique often comes down to the researcher’s familiarity with...the nature of the experiment, it is up to the researcher to determine which method is most appropriate...stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...
  11. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...
  12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post
    ... Resources on Addgene.org Search for AAV Vectors to use with your research. Visit Our AAV Guide Page ...a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating... new AAVs can serve as tools for basic science research (neuronal tracing and morphologic studies), as...
  13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...experiments that work anywhere in the genome. Aneesh's research interests include proteomics, machine learning...Taking a nerdy perspective, you might denote your search as follows: (my_house + coffee) - starbucks See...Data. Quilt is a collaborative database. Aneesh's research interests span machine learning, proteomics, and... on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find Validated gRNAs...
  14. Using AAV for Neuronal Tracing

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    Blog Post
    ...understanding the brain’s function is knowing its architecture, in particular the connections between different...to their advantageous characteristics for brain research and their ability to introduce genetic material...fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different AAV vectors...Atlas website. A similar approach was used by researchers from the Janelia Campus for their MouseLight ...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2...
  15. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...
  16. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post
    ...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...
  17. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...signal at a point on the axis determined by the researcher, based on their controls. You can usually tell...intimated if you’re struggling to understand how the researchers decided to place their gates on the plots! Flow...gating strategy, you can start to ask why the researchers made the decisions they did in their experiments...vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom...
  18. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post
    ...and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124...cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332...enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713...the CRISPR guide Find CRISPR plasmids for your research ...
  19. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post
    ...the College of Life Sciences that same year. Her research is focused on the escape mechanism of genome editing...Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-encoded...professor in Synthetic Biology in 2006. The Yang lab's research is focused on developing microbial genome editing...Sciences in September 2019 as a Ph.D. candidate. Her research focuses mainly on the development and application...
Showing: 441 - 460 of 923 results