...Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an interesting...to check the library information and original publication to see if a library is suitable for your experiment...specified by the gRNA plus the genomic PAM sequence Publications (by topic) CRISPR basics Anders, C., Niewoehner.... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

... functional heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous...

... the gRNA and scaffold. If you don’t feel like doubling the amount of cloning, another option is to use...

...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ...

...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...

... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

...This approach can augment viral surveillance by enabling rapid assessment of antigenic drift.    Starr...

... pursue a career in science communication and publishing after completing her postdoctoral training from...

... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

...can be calibrated to these different conditions, enabling the use of a given timer in many experimental ...

... induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional benefit of the use of...

...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

...with techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture...

... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...

...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier...

...PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug...

...bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging ...

...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...experiment or evaluating ExM images in a new publication.         Why Expansion Microscopy?  Traditional...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...