We narrowed to 451 results for: CRISPR

1. Adeno-associated virus (AAV) Guide

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   Guide

   ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...

2. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...

3. How to Deposit Your Plasmids with Addgene

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   Blog Post

   ...sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs...

4. Antibody Validation for Flow Cytometry

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   Blog Post

   ...expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO...

5. Adenovirus Guide

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   Guide

   ...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

6. Viral Vectors 101: Systemic Capsids

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   Blog Post

   ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...

7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...

8. Fluorescent Proteins 101: Fluorescent Biosensors

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   Blog Post

   ...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...

9. Tetracycline Inducible Expression

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   Collection

   ...expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1...-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9...

10. Neurodegeneration Plasmid Collection

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    Collection

    ...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 ...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type ...LRRK2 His T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi...pET-Duet_OPTN-thrombin-GST OPTN GST T7 ALS Sascha Martens 218527 plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA EIF2AK2 U6 VWM disease...

11. Immunology Research Plasmids and Resources

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    Collection

    ... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene...