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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 802 results for: Clu

Showing: 441 - 460 of 802 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...are needed to optimize yield. You might want to include an extra wash step or use a different strain. For.... Look out for carbohydrates Certain strains, including HB101 and derivatives, release large amounts of...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...their production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a ...IgG subclasses, so that secondary antibodies exclusively recognize only one primary antibody. This process...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...in the detection of microbes. Some applications include measuring viable probiotics and detecting circulating...

  6. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported...al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated purification of a specific genomic...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...One of the cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    ...– it makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and...which have fragments of the AdV genome in them, including the E1 region). The space in the viral particle...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...will simply express from whatever promoters were included in the vector (or are nearby in the host genome...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...the primer sequences. Multiple plasmids can be included in a single PCR, for example for subcloning genes...vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning - To...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    ...accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, ...combinations, though frequently those combinations still include at least one element from the original TAP tag....

  14. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...to the total number of viral capsids (which can include empty capsids that are devoid of a viral genome... et al., 2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which...

  15. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... in the linings of certain areas of the body, including the lung, the heart, and the abdomen. Long story...primary conventional treatments for mesothelioma include surgery to remove cancerous masses (tumor resection...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibiotic resistance develop? Microbes (bacteria included) are very skilled at the delicate art of survival... contributing to the development of resistance include (Figure 1): Over-prescription of antibiotics (...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...method can be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection...Cas12a is initiated. Surrounding trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded...

  18. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... works on our site. For Addgenies, development includes everything from tracking plasmids from the moment...then go back to this area later and tidy it up. Conclusion While none of us are life science majors, we ...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...target the ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein...mScarlet-I3 that were codon-optimized for C. elegans and included artificial introns to improve expression. When...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...show your enthusiasm for getting the position and include a brief summary of why you are a good applicant...taught for five years at [name of the university]” Include keywords from the job description to help the hiring...
Showing: 441 - 460 of 802 results