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We narrowed to 772 results for: Dos

Showing: 441 - 460 of 772 results

  1. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...life. That’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would...to express your protein of interest. However, you do still need a species-compatible promoter for expressing...

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...five of these Cas proteins, while type I-C Cascades do not contain Cas6. Regardless of the subtype, the ...to the multi-Cas mechanism. These benefits of Cas3 do come at a cost — the previously mentioned issues ...

  3. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...

  4. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluoresce, the Jaffrey lab asked:  Could an RNA aptamer do the same thing? Using the HBI derivative 3,5-difluoro...recognition of ions or particularly small molecules that do not generate an immune response. Aptamers can be ...

  5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...negative control to determine whether your PIPs actually do interact. Choosing the right negative control is ...scenarios that call for a simple, yes-no answer — i.e., do two proteins interact or not? — FRET is preferable...endogenous PIPs should decrease the BiFC signal in a dose-dependent manner. This is straightforward in easy-to-transfect...easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also theoretically...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...gradients, practice making the iodixanol layers before doing your first purification. This helped me get a feel...to learn more about different ways to titer AAV. Do you have any AAV production tips or tricks? Please...multiplicity of infections (MOIs), to determine the optimal dose of AAV for my cells of interest. Every batch of ...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...offer tools to help with these decisions.  So when do you want to codon optimize your gene of interest?...useful in expressing functional proteins in hosts that do not naturally express that gene. The protein you ...

  10. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...expression. To design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators... lentiviral system (Figure 2). The HIV genes that do remain are very important for viral production: Gag...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

  13. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more about...

  15. Deck the Lab 2022!

    Type
    Blog Post
    ... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

  17. Editor's Choice, July 2016

    Type
    Blog Post
    ...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

  19. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...
Showing: 441 - 460 of 772 results