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Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

Sarting a journal club? Read our guide to the logistics of forming a journal club and find different formats to hold the journal club discussion.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

Jim Woodgett discusses the data sharing policies of journals, such as PLOS, and how access to data affects scientific research.

It's time to announce the winner of our annual #DeckTheLab competition!

Master the streak plate technique using a mixture of E. coli strains expressing a different chromoprotein.

This post describes the development of sentinel plants, which change color to alert humans when they detect certain molecules in their environment.

Ghanaian researcher, Kwabena Duedu discusses his work in public health, synthetic biology, and microbiology.

Blog editor, Tyler J. Ford, summarizes the top post from July 2016 and picks another post that he thinks deserves another look.

The basics of Ebola virus and links to helpful resources for scientists interested in studying Ebola.

Expansion microscopy allows for light microscope imaging of fixed tissues while overcoming the 250 nm resolution limit.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.