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We narrowed to 769 results for: Dos

Showing: 441 - 460 of 769 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...gradients, practice making the iodixanol layers before doing your first purification. This helped me get a feel...to learn more about different ways to titer AAV. Do you have any AAV production tips or tricks? Please...multiplicity of infections (MOIs), to determine the optimal dose of AAV for my cells of interest. Every batch of ...

  3. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...expression. To design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators... lentiviral system (Figure 2). The HIV genes that do remain are very important for viral production: Gag...

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

  7. Deck the Lab 2022!

    Type
    Blog Post
    ... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

  9. Editor's Choice, July 2016

    Type
    Blog Post
    ...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

  10. Guide to Using Pooled Libraries

    Type
    Guide
    ...: Apply library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort... screen, the goal is to identify those cells that do not survive the selection mechanism. You’ll infect...

  11. Antibody Guide

    Type
    Guide
    ...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...

  13. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...

  14. Molecular Biology Reference

    Type
    Guide
    ...function and the correct gene of interest. So how do scientists sequence DNA? Sanger Sequencing In 1975...fluorescently tagged nucleotide. Because these special bases do not have a binding site for adding the next nucleotide... can be converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does...is important to note that NGS platforms in general do not require a specific primer for your DNA of interest...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

  19. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...
Showing: 441 - 460 of 769 results