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We narrowed to 805 results for: Eng

Showing: 441 - 460 of 805 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...enabling a broad spectrum of donor fluorescence wavelengths for FRET experiments. However, remember that,...amino acid at a stop codon (often amber i.e. UAG) engineered into a desired site. During translation, the ...molecules to each Cy dye, as well as by extending the length of the histidine tag from 6 to 10 residues. While...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorescent signal that is captured by detectors. The strength of the emitted photons varies from cell to cell...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...

  5. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. ...CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of CRISPR, Addgene...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    ...temperature reduces background noise but increases the length of your assay. The most common options are 4 °C...background noise as well, and multiplexing is challenging and limited. Direct westerns typically rely on...waste. However, they also add time to an already lengthy protocol, require the use of hazardous chemicals...

  8. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...-defined contours. (Many of them are software engineers, surprisingly, who have deep technical knowledge...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog...

  9. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  10. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

  12. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...sciencewithtom.com.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  13. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  14. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  15. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,...

  18. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...
Showing: 441 - 460 of 805 results