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We narrowed to 802 results for: Eng

Showing: 441 - 460 of 802 results

  2. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. ...CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of CRISPR, Addgene...

  3. Technical Design of a Western Blot

    Type
    Blog Post
    ...temperature reduces background noise but increases the length of your assay. The most common options are 4 °C...background noise as well, and multiplexing is challenging and limited. Direct westerns typically rely on...waste. However, they also add time to an already lengthy protocol, require the use of hazardous chemicals...

  5. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...-defined contours. (Many of them are software engineers, surprisingly, who have deep technical knowledge...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog...

  6. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  7. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

  8. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...sciencewithtom.com.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  10. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  11. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  12. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...

  14. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,...

  17. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

  19. Let There Be LITE Plasmids

    Type
    Blog Post
    ...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...

  20. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments.  Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ...
Showing: 441 - 460 of 802 results