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Showing: 441 - 460 of 1141 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400...deposited the associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more...with Addgene causes a paper to get cited more?   Articles with and without plasmids at Addgene To ...authors deposited with Addgene and those without plasmids deposited with Addgene. Together these journals...of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what... at Addgene – typically about four times as many as papers without plasmids deposited with Addgene (Figure...

  2. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... in most cases at Addgene, we check what we call the “insert,” (the gene or genetic element cloned into...information for a given plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...

  4. Addgene's Top 10 Blog Posts of 2015

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    Blog Post
    ... researchers with many different ways to control gene expression. Get the full scoop from Addgenie A. ...how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6. CRISPR 101: Non-homologous...Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have limited packaging capacity making...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...your own guest posts by e-mailing us at blog@addgene.org. Thank you again for a fantastic year and happy...

  5. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers, so Outreach Scientist...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As ...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation should...and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The...considerations you should think about when developing optogenetics behavioral experiments. There are far too many...many behaviors that have utilized optogenetics to be fully summarized in a short blog post, but some examples...ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type...the literature to find all the applications of optogenetics relevant to your field. Ultimately, your question...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ... development of optogenetics, has genetically manipulated a number of the opsin genes to alter the light...have also been engineered to express various opsin genes [7-9]. The advantage of using these lines is, if...Regardless of the method of expression of your opsin gene, it is essential to test the expression of your ...PMID: 22470147.  6. Gore BB, et al. Manipulating gene expression in projection-specific neuronal populations...sections of optogenetics papers. Optogenetics step one: Deciding how you want to use optogenetics The most...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and, ...electrophysiology to prove that your optogenetic manipulations are generating action potentials (or lack there...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...Hey guys, Blugene here! As Addgene’s mascot, I’ve watched Addgene grow into the successful, internationally...to the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the... the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and...changes at Addgene over the last 10 years as well as the many people that make up the Addgene family. It...Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination...make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering ...reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition of standards...antibiotic resistance markers, promoters, and inducible gene expression systems in bee gut microbiome species...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the... also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take a look ...microbiome with sequencing is vast, the ability to genetically alter microbiome members is limited and many ...

  10. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    .... Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an...pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating ...performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current...adult rat brain depending on the transduced area. Gene Ther. 2003 Feb;10(4):337-47. PubMed PMID: 12595892...Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...accelerate their work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses...please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools,...

  11. Lucky Thirteen: Guest Contributors of 2023

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    Blog Post
    ... PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl;...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...

  12. Antibodies 101: Validation

    Type
    Blog Post
    ... your antibody is validated! Genetic Strategies This approach uses a gene knock-down or knock-out approach...all sample types are amenable to genetic manipulation, nor can all genes be easily knocked down or out. ...bioinformatician) to look at RNA expression of your gene of interest across a set of human tissues.  From...Conveniently, you found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9...plasmid encoding your protein of interest, known as Gene of Interest (GOI), and transfect it into your favorite...this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run a Western... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...

  13. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post
    ...Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning Sytem to Clone Your Gene of Interest...making it difficult to quickly identify possible gene function. In this post, I will describe a useful...done, this technique can also help pinpoint the genes within a sequenced genome that encode for functional...digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these fragments can be ...results in optimal likelihood of capturing a whole RBP gene without causing issues associated with cloning large...back to the original plate to identify which phage gene encodes that protein! First, block your membranes...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...

  14. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    .... Current table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated...reviewed articles. If you’re targeting your favorite gene with CRISPR, using one of these validated gRNAs ...is worth trying alternate names, particularly for gene names. If you find a sequence that will work for... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop... is welcomed for validated gRNAs that are not generated from a plasmid. If you, for example, prefer to...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...

  15. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

  17. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

  18. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the... with the recipient organization can create an Addgene account and place an order: a purchasing agent,...

  19. Meet Aavery, Blugene's New Virus Friend!

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    Blog Post
    ...Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus...March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name! We thank ...we’re excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like...

  20. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...
Showing: 441 - 460 of 1141 results