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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: LIS;

Showing: 441 - 460 of 772 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

  2. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

  6. March for Science

    Type
    Blog Post
    ...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...

  10. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ...

  12. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for ...like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

  18. Finding Your Perfect Job After University

    Type
    Blog Post
    ...duration of only three months and made a good job to list on my CV. Three months was a perfect duration for...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...

  19. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

  20. A Control for All Seasons

    Type
    Blog Post
    ....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...
Showing: 441 - 460 of 772 results