We narrowed to 768 results for: LIS;

...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...Experiment with New Careers while in Grad School Listen to Michael Koeris' Journey from Grad Student to...

...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly...

...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...

...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ...

... to the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another...

...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...

...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

...especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for ...like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing...

..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to...

...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...

...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

... Twitter. Be ready for the interview with a long list of written questions for the PI and lab members....too, they may not have had experience writing, publishing, or discussing career opportunities with the ...

...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the presence...wide range of detection capabilities. See a full list of detection solutions and HiBiT products offered...

...new empty backbones By Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's...the payload sequence into the genome. The team established that they could reprogram the genomic target ...