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We narrowed to 902 results for: Nes

Showing: 441 - 460 of 902 results

  1. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...directions of the project, Outlines a detailed scope of specific aims, milestones, possible setbacks, and...will address these limitations and lead to new milestones in the field. Research strategy The Research...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...

  3. Water Bath Protocol

    Type
    Protocol
    ...digests needed in molecular biology, and thaw cell lines. There are many different types of water baths that...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...long-term solution to reproducibility and cost-effectiveness for broad and large quantity uses. We therefore...accuracy while screening. We also compare different clones of the same target to see if the results are the...primary antibody compared to negative control also determines the signal’s strength. Exposure time is the amount...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...spike protein can be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus...diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase and Taq..., from Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung... information on these plasmids and a behind-the-scenes glimpse of their work, head over to their blog ...

  6. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...in your career path and still find comfort and happiness there. Read all of the Meet an Addgenies features...Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant Biochemistry...she joined an investment club and discovered the business side of science, she stuck with it. Lab Manager...people, and making them get outside of their comfort zones. I came to Addgene with a goal to learn nonprofit...

  7. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...cells, one may have the unfortunate experience of witnessing…nothing. For some unclear reason, cells are not...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic...conditions for storage, transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... such as LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers prefer...that target the common features of viral vector backbones; once validated these universal primers can be...popular approach utilizes the antibiotic resistance genes carried on transfer plasmids. Target cells are transduced... for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...molecular biology toolbox to identify new target genes for drug discovery or to understand the intricacies...the targeting of hundreds to tens of thousands of genes with minimal effort. The versatility of Cas effectors...constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens may be AAVantageous...). Next-generation sequencing (NGS) to identify genes of interest. Figure 1: Overview of in vitro...

  10. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...other, you should be able to draw perpendicular lines that intersect your populations. If you see a diagonal...needs to be shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal... Inputting compensation values differs between machines or the program you’re using, but usually you can... the plot. However, because we can see straight lines in the populations in the other corners of the dot...

  11. Transferable Skills: Negotiation

    Type
    Blog Post
    ...wearing a lab coat, but instead by people wearing business suits discussing multimillion dollar contracts... or coursework. First, you have to develop an awareness of when you are negotiating. Then take a moment...situation instead of just extra work for them? As a business development analyst, I negotiate with global organizations...professor or signing contracts when working for a business development team, negotiation is a key transferable...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat severe...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue their work by...CRISPR-based lineage tracing tool captures rare clones that represent 0.001% of the population.  Because...CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression ...

  13. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...extra clips to hold up your poster, and pack a few business cards to tack up next to your poster. Arrive early... won’t know every answer – don’t hesitate to be honest and tell your audience just that. Explain what ...around the posters and read the titles to find the ones you’d like to revisit. Strategically traverse the...with the presenter after the session. Exchange business cards or jot down their name and affiliation from...

  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...and prepare business cards. You might not consider being a student or postdoc a “business,” but having...having business cards is a good way to share your information quickly. During the conference Arriving at the...myself like meet ten new people each day. Exchange business cards. When you’re done networking for the day...

  15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...H840. To ensure that the clones were capable of cleavage, they tested the clones against an NNN PAM library...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...variants recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition...

  16. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... do everything as I planned and I was missing deadlines - which puzzled me because I could do all these...basis! The silence and maybe even the slight awkwardness can spur people to speak up. Once aware of the...to be!  References Becoming the boss. Harvard Business Review, January 2007. Three Rules for Managing...  Are Good Managers Born or Made? UPenn Wharton Business School Blog Additional Resources on the Addgene...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown... al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue),...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

  18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...cells by the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...C.A., et al., Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(...Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....

  19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully ...used over and over again in combination with novel genes to get very precise expression levels. The BIOFAB... for someone to forward engineer a system of 10 genes. You can now forward engineer a 10-gene system to...our testing of these parts that the chance your genes will be expressed at the level you want is 93 percent...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary... depending on whether the heavy and light chain genes are contained to one plasmid or if they’ve been ...have multiple contaminating bands or a few strong ones, consider increasing the number of washes performed...
Showing: 441 - 460 of 902 results